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Protocol
Profiling of Short RNAs Using Helicos Single-Molecule Sequencing
The importance of short (<200 nt) RNAs in cell biogenesis has been well documented. These short RNAs include crucial classes of molecules such as transfer RNAs, small nuclear RNA, microRNAs, and many others (r...
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Protocol
Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing
Methods for in-depth characterization of transcriptomes and quantification of transcript levels have emerged as valuable tools for understanding cellular physiology and human disease biology, and have begun to...