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    Protocol

    Profiling of Short RNAs Using Helicos Single-Molecule Sequencing

    The importance of short (<200 nt) RNAs in cell biogenesis has been well documented. These short RNAs include crucial classes of molecules such as transfer RNAs, small nuclear RNA, microRNAs, and many others (r...

    Philipp Kapranov, Fatih Ozsolak in Next-Generation MicroRNA Expression Profil… (2012)

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    Protocol

    Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing

    Methods for in-depth characterization of transcriptomes and quantification of transcript levels have emerged as valuable tools for understanding cellular physiology and human disease biology, and have begun to...

    Fatih Ozsolak, Patrice M. Milos in High-Throughput Next Generation Sequencing (2011)