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  1. No Access

    Protocol

    Quantitative Polyadenylation Site Map** with Single-Molecule Direct RNA Sequencing

    The known regulatory role of 3′ untranslated regions (3′UTRs) and poly(A) tails in RNA localization, stability, and translation, and polyadenylation regulation defects leading to human diseases such as oculoph...

    Fatih Ozsolak in Polyadenylation (2014)

  2. No Access

    Protocol

    Profiling of Short RNAs Using Helicos Single-Molecule Sequencing

    The importance of short (<200 nt) RNAs in cell biogenesis has been well documented. These short RNAs include crucial classes of molecules such as transfer RNAs, small nuclear RNA, microRNAs, and many others (r...

    Philipp Kapranov, Fatih Ozsolak… in Next-Generation MicroRNA Expression Profil… (2012)

  3. No Access

    Article

    RNA sequencing: advances, challenges and opportunities

  4. Powered by improving next-generation sequencing capabilities, RNA-seq studies continue to provide knowledge about the quantitative and qualitative aspects of t...

    5. Fatih Ozsolak, Patrice M. Milos in Nature Reviews Genetics (2011)

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    Protocol

    Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing

    Methods for in-depth characterization of transcriptomes and quantification of transcript levels have emerged as valuable tools for understanding cellular physiology and human disease biology, and have begun to...

    Fatih Ozsolak, Patrice M. Milos in High-Throughput Next Generation Sequencing (2011)