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  1. No Access

    Article

    Partitioning of Recombinant Pseudomonas putida POS-F84 Proline Dehydrogenase in Aqueous Two-Phase Systems: Optimization Using Response Surface Methodology

    Empirical modeling the partition behavior and recovery of a recombinant Pseudomonas putida POS-F84 proline dehydrogenase (ProDH) in aqueous two-phase systems (ATPS) was carried out by response surface methodology...

    Eskandar Omidinia, Hamid Shahbazmohammadi… in Applied Biochemistry and Biotechnology (2019)

  2. No Access

    Article

    Engineering an efficient mutant of Eupenicillium terrenum fructosyl peptide oxidase for the specific determination of hemoglobin A1c

    Fructosyl peptide oxidase (FPOX, EC 1.5.3) belongs to the family of oxidoreductases, which is used as a diagnostic enzyme for diabetes mellitus. FPOX has activities toward Fru-ValHis and Fru-Lys as model compo...

    Hamid Shahbazmohammadi, Soroush Sardari… in Applied Microbiology and Biotechnology (2019)

  3. No Access

    Article

    Medium Optimization for Improved Production of Dihydrolipohyl Dehydrogenase from Bacillus sphaericus PAD-91 in Escherichia coli

    Dihydrolipohyl dehydrogenase (DLD) is a FAD-dependent enzyme that catalyzes the reversible oxidation of dihydrolipoamide. Herein, we report medium optimization for the production of a recombinant DLD with NADH...

    Hamid Shahbazmohammadi, Eskandar Omidinia in Molecular Biotechnology (2017)

  4. No Access

    Article

    Purification and Characterization of Recombinant Darbepoetin Alfa from Leishmania tarentolae

    Darbepoetin alfa is a biopharmaceutical glycoprotein that stimulates erythropoiesis and is used to treat anemia, which associated with renal failure and cancer chemotherapy. We herein describe the structural c...

    Anvarsadat Kianmehr, Abdolkarim Mahrooz, Morteza Oladnabi… in Molecular Biotechnology (2016)

  5. No Access

    Article

    Aptamer-based Biosensor for Detection of Phenylalanine at Physiological pH

    A simple, sensitive aptamer-based biosensor for the detection of phenylalanine is developed using the electrochemical transduction method. For this proposed aptasensor, a 5-thiol-terminated aptamer is covalent...

    Eskandar Omidinia, Nasrin Shadjou… in Applied Biochemistry and Biotechnology (2014)

  6. No Access

    Article

    Investigation of chromatography and polymer/salt aqueous two-phase processes for downstream processing development of recombinant phenylalanine dehydrogenase

    This work presents a comprehensive study between the polymer/salt aqueous two-phase systems (ATPS) and chromatography process for downstream processing of recombinant Bacillus badius phenylalanine dehydrogenase (...

    Eskandar Omidinia, Hamid Shahbaz Mohamadi… in Bioprocess and Biosystems Engineering (2010)

  7. No Access

    Article

    Differential expression of human basic fibroblast growth factor in Escherichia coli: potential role of promoter

    Four different expression systems were developed for expression of the cDNA encoding human basic fibroblast growth factor (hbFGF), using Escherichia coli as host organism. The hbfgf structural gene was cloned int...

    Hasan Mirzahoseini, Farideh Mehraein… in World Journal of Microbiology and Biotechn… (2004)