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Open AccessNew indicators and indexes for benchmarking university–industry–government innovation in medical and life science clusters: results from the European FP7 Regions of Knowledge HealthTIES project
While the European Union is striving to become the ‘Innovation Union’, there remains a lack of quantifiable indicators to compare and benchmark regional innovation clusters. To address this issue, a HealthTIES...
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Personal Data Cooperatives – A New Data Governance Framework for Data Donations and Precision Health
Personalized health research depends on aggregated sets of personal data from millions of people. Given that personal data can be copied, individuals are entitled to copies of their data and individuals are th...
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Article
Open AccessDemocratizing Health Research Through Data Cooperatives
Massive amounts of data are collected and stored on a routine basis in virtually all domains of human activities. Such data are potentially useful to biomedicine. Yet, access to data for research purposes is h...
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Article
Open AccessProteome-wide association studies identify biochemical modules associated with a wing-size phenotype in Drosophila melanogaster
The manner by which genetic diversity within a population generates individual phenotypes is a fundamental question of biology. To advance the understanding of the genotype–phenotype relationships towards the ...
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Article
Open AccessThe Lnk/SH2B adaptor provides a fail-safe mechanism to establish the Insulin receptor-Chico interaction
Insulin/insulin-like growth factor signalling (IIS) has been described as one of the major pathways involved in growth control and homeostasis in multicellular organisms. Whereas its core components are well e...
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Article
Open AccessUse of anionic denaturing detergents to purify insoluble proteins after overexpression
Many proteins form insoluble protein aggregates, called “inclusion bodies”, when overexpressed in E. coli. This is the biggest obstacle in biotechnology. Ever since the reversible denaturation of proteins by chao...
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Article
Open AccessThe lack of autophagy triggers precocious activation of Notch signaling during Drosophila oogenesis
The proper balance of autophagy, a lysosome-mediated degradation process, is indispensable for oogenesis in Drosophila. We recently demonstrated that egg development depends on autophagy in the somatic follicle c...
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Article
Open AccessMadm (Mlf1 adapter molecule) cooperates with Bunched A to promote growth in Drosophila
The TSC-22 domain family (TSC22DF) consists of putative transcription factors harboring a DNA-binding TSC-box and an adjacent leucine zipper at their carboxyl termini. Both short and long TSC22DF isoforms are ...
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Article
Open AccessThe Drosophila melanogaster PeptideAtlas facilitates the use of peptide data for improved fly proteomics and genome annotation
Crucial foundations of any quantitative systems biology experiment are correct genome and proteome annotations. Protein databases compiled from high quality empirical protein identifications that are in turn b...
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Open AccessImp-L2, a putative homolog of vertebrate IGF-binding protein 7, counteracts insulin signaling in Drosophila and is essential for starvation resistance
Insulin and insulin-like growth factors (IGFs) signal through a highly conserved pathway and control growth and metabolism in both vertebrates and invertebrates. In mammals, insulin-like growth factor binding ...
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Open AccessBunched, the Drosophilahomolog of the mammalian tumor suppressor TSC-22, promotes cellular growth
Transforming Growth Factor-β1 stimulated clone-22 (TSC-22) is assumed to act as a negative growth regulator and tumor suppressor. TSC-22 belongs to a family of putative transcription factors encoded by four di...
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Article
Open AccessA universal method for automated gene map**
Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genoty** method that relies on the d...
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Article
Open AccessIntegration with the human genome of peptide sequences obtained by high-throughput mass spectrometry
A crucial aim upon the completion of the human genome is the verification and functional annotation of all predicted genes and their protein products. Here we describe the map** of peptides derived from accu...
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Article
Open AccessA reverse genetic screen in Drosophila using a deletion-inducing mutagen
We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible...
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Open AccessThe Drosophila Forkhead transcription factor FOXO mediates the reduction in cell number associated with reduced insulin signaling
Forkhead transcription factors belonging to the FOXO subfamily are negatively regulated by protein kinase B (PKB) in response to signaling by insulin and insulin-like growth factor in Caenorhabditis elegans and m...
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Wing commanders
