17 Result(s)
within
Da-Neng Wang
English
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Article
Open AccessProton-coupled transport mechanism of the efflux pump NorA
Efflux pump antiporters confer drug resistance to bacteria by coupling proton import with the expulsion of antibiotics from the cytoplasm. Despite efforts there remains a lack of understanding as to how acid/b...
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Article
Structural basis for inhibition of the drug efflux pump NorA from Staphylococcus aureus
Membrane protein efflux pumps confer antibiotic resistance by extruding structurally distinct compounds and lowering their intracellular concentration. Yet, there are no clinically approved drugs to inhibit ef...
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Open AccessStructural basis of ion – substrate coupling in the Na+-dependent dicarboxylate transporter VcINDY
The Na+-dependent dicarboxylate transporter from Vibrio cholerae (VcINDY) is a prototype for the divalent anion sodium symporter (DASS) family. While the utilization of an electrochemical Na+ gradient to power su...
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Article
Structure and inhibition mechanism of the human citrate transporter NaCT
Citrate is best known as an intermediate in the tricarboxylic acid cycle of the cell. In addition to this essential role in energy metabolism, the tricarboxylate anion also acts as both a precursor and a regul...
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Article
Open AccessKe Hsin Kuo: A distinguished scientist and great mentor
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Article
ATP binding drives substrate capture in an ECF transporter by a release-and-catch mechanism
Bacterial energy-coupling factor (ECF) transporters mediate ATP-dependent uptake of essential environmental micronutrients. Biochemical and fluorescence analyses now show that ATP binding promotes release of a...
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Structure and mechanism of a bacterial sodium-dependent dicarboxylate transporter
The cytosolic concentration of citrate partially depends on its direct import across the plasma membrane by the Na+-dependent citrate transporter (NaCT); here the X-ray crystal structure of a bacterial homologue ...
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Article
Identification and characterization of a bacterial hydrosulphide ion channel
A channel for the transport of hydrosulphide ions in Clostridium difficile is identified and shown to be polyspecific, being a member of the formate/nitrite transporter family.
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Article
The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins
The New York Consortium on Membrane Protein Structure (NYCOMPS) was formed to accelerate the acquisition of structural information on membrane proteins by applying a structural genomics approach. NYCOMPS compr...
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Article
Transporter in the spotlight
Membrane transporter proteins switch between conformational states to move substrates across membranes. The transition between these states can now be studied using single-molecule experiments.
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Article
Structure and mechanism of a pentameric formate channel
The structure of the formate channel FocA from Vibrio cholerae reveals a pentamer, with each monomer possessing its own substrate translocation pore. The selectivity filter consists of a central constriction ring...
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Article
Antidepressant specificity of serotonin transporter suggested by three LeuT–SSRI structures
Sertraline (Zoloft) and fluoxetine (Prozac) are selective serotonin-reuptake inhibitors (SSRIs) that are widely prescribed to treat depression. They bind to the presynaptic plasma membrane serotonin transporte...
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Article
Three-dimensional Architecture of Hair-bundle Linkages Revealed by Electron-microscopic Tomography
The senses of hearing and balance rest upon mechanoelectrical transduction by the hair bundles of hair cells in the inner ear. Located at the apical cellular surface, each hair bundle comprises several tens of...
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Article
Electron microscopic analysis of KvAP voltage-dependent K+ channels in an open conformation
Voltage-dependent ion channels serve as field-effect transistors by opening a gate in response to membrane voltage changes1. The gate's response to voltage is mediated by voltage sensors2, which are arginine-cont...
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Protocol
Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrane
Functions and biochemical properties of several membrane transporter proteins from human erythrocyte, in particular, the glucose transporter (Glut1) and anion exchanger (AE1, also called Band 3) have been exte...
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Article
Atomic model of plant light-harvesting complex by electron crystallography
The structure of the light-harvesting chlorophyll a/b–protein complex, an integral membrane protein, has been determined at 3.4 Å resolution by electron crystallography of two-dimensional crystals. Two of the thr...
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Article
Three-dimensional structure of plant light-harvesting complex determined by electron crystallography
The structure of the light-harvesting chlorophyll a/b-protein complex, a membrane protein serving as the major antenna of solar energy in plant photosynthesis, has been determined at 6 Å resolution by electron cr...
