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Chapter and Conference Paper
Cytokinin Function in Drought Stress Response and Subsequent Recovery
Plants respond to exogenous stimuli, including abiotic stress, by modulation of their behavior, which is partially mediated by plant hormones. Understanding of the role of different hormones could improve the ...
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Article
Substrate specificity and domain analyses of zeatin O-glycosyltransferases
The substrate specificity of two recombinant enzymes, zeatin O-glucosyltransferase 1 (ZOG1) and zeatin O-xylosyltransferase 1 (ZOX1), was further characterised. ZOG1 utilises zeatin (Z), UDPG, and UDPX as subs...
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Article
Genes Encoding Zeatin O-Glycosyltransferases
Zeatin is a highly active and ubiquitous cytokinin. O-Glycosylation of zeatin occurs widely in plant tissues and is likely to be important in regulating the level of active cytokinins. Enzymes mediating the co...
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Article
Analyses of Phaseolus vulgaris L. and P. coccineus Lam. hybrids by RFLP: preferential transmission of P. vulgaris alleles
Restriction fragment length polymorphism (RFLP) was determined among P. vulgaris genotypes and Phaseolus species using 19 probes. The incidence of polymorphism was high (70–86%) between species, but relatively lo...
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Article
Somatic embryogenesis and shoot organogenesis from interspecific hybrid embryos of Vigna glabrescens and V. radiata
Somatic embryogenesis occurred on cotyledons of morphologically abnormal embryos derived from Vigna glabrescens x V. radiata crosses and cultured on Murashige and Skoog (MS) medium without growth regulators. The ...
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Chapter and Conference Paper
Genetic Differences in the Enzymatic Regulation of Zeatin Metabolism in Phaseolus Embryos
Hormones play a significant role in all phases of growth and development of higher plants. Although the site(s) of action of hormones are largely unknown, it can be postulated that rather precise regulatory me...
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Article
Interspecific hybridization between Vigna radiata (L.) Wilczek and V. glabrescens
Interspecific hybrids of the mungbean, Vigna radiata (L.) Wilczek (2n=22) and V. glabrescens (2n=44) were generated with the aid of embryo culture. V. glabrescens x V. radiata hybrids were recovered via germinati...
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Chapter
Phaseolus: Wide Hybridization Through Embryo Culture
Grain legumes are second only to cereals as important plant food sources. The cultivation of grain legumes amounts to over 72 million ha, with a total production of about 51 million metric tons per year (FAO P...
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Article
Embryo development in reciprocal crosses of Phaseolus vulgaris L. and P. coccineus Lam.
Embryo development was examined in reciprocal crosses of Phaseolus vulgaris cv. ‘Great Northern’ and P. coccineus cv. ‘Scarlet Runner’. The formation of abnormal (shrunken and underdeveloped) embryos constituted ...
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Chapter and Conference Paper
Genetic Regulation of Cytokinin Metabolism in Phaseolus Tissue Cultures
The regulatory mechanisms controlling hormonal metabolism play a central role in plant development. For normal development to occur, hormone levels appropriate to each tissue at each stage of development must ...
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Article
Meiosis and fertility of interspecific hybrids betweenPhaseolus vulgaris L. andP. acutifolius A. Gray
Meiosis and fertility of interspecific hybrids obtained from reciprocal crosses betweenPhaseolus vulgaris andP. acutifolius were examined. Bivalents as well as univalents were found at Metaphase I. The majority o...
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Article
Meiosis and fertility of interspecific hybrids between Phaseolus vulgaris L. and P. acutifolius A. Gray
Meiosis and fertility of interspecific hybrids obtained from reciprocal crosses between Phaseolus vulgaris and P. acutifolius were examined. Bivalents as well as univalents were found at Metaphase I. The majority...
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Article
Fertilization and early embryo development in reciprocal interspecific crosses of Phaseolus
Fertilization and early embryo and endosperm development were examined in Phaseolus vulgaris x P. acutifolius, P. vulgaris x P. lunatus crosses and their reciprocals. The number and length of pollen tubes were no...
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Article
Interspecific hybridization of phaseolus vulgaris with P. lunatus and P. acutifolius
The influence of genotypic combinations on the growth of hybrid embryos between Phaseolus vulgaris and P. lunatus, and between P. vulgaris and P. acutifolius was examined. All embryos obtained from P. vulgaris ×
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Article
Monosomics in common bean, Phaseolus vulgaris
Two monosomics of Phaseolus vulgaris (2n = 22) were found among selfed progeny of plants treated with colchicine. The monosomic chromosomes involved were identified as chromosomes H and J according to the previou...
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Article
Genetic evidence for mode of 2n pollen formation andS-locus map** in potatoes
Two diploid clones heterozygous for the geney (yellow tuber flesh), and producing diplandroids (2n pollen) by parallel spindles (ps) and premature cytokinesis 1 (pc1), respectively, were crossed to eight white-fl...
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Article
The inheritance of three mechanisms of diplandroid (2n pollen) formation in diploid potatoes
Genetic data obtained from seven test-crosses (159 progeny) substantiated an earlier hypothesis that all three mechanisms of diplandroid formation (parallel spindles, ps; premature cytokinesis-1, pc-1 and prematu...
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Article
Cytology of potato triploids producing 2N pollen
Parallel spindles (ps) were found in a spontaneously occurring triploid plant ofS. chacoense and two triploids ofS. commersonii. This may be the basis for the formation of plump, stainable pollen in these triploi...
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Article
Breeding value of 2n pollen (diplandroids) in tetraploid x diploid crosses in potatoes
The relative breeding value of first division restitution (FDR) and second division restitution (SDR) 2n male gametes from diploids, and n (2x) gametes from tetraploids were compared. This was done by measurin...
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Article
Identification of potato chromosomes with Giesma
A modified Giesma staining technique was used to identify the somatic chromosomes of diploid potatoes. Distinct banding patterns were observed on all 12 chromosomes. Individual chromosomes were identified acco...