1,296 Result(s)
within
Chen Yu-**
Life Sciences
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Article
A new and simplified method for the determination of ergot alkaloids
The conventional method for determining ergot alkaloids in fermentation media is laborious and time consuming, largely because of the chloroform extraction procedure employed. A new and simplified method, with...
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Article
Mutation(s) necessary for the residence of RP4 inXanthomonas campestris pv.Citri
The broad-host-range plasmid RP4 was conjugatively transferred fromEscherichia coli toXanthomonas campestris pv.citri strain XAS4501. Low transfer frequency of RP4 (about 6.2×10−7) intoXanthomonas and deletion of...
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Article
Identification of anodD-like gene inFrankia by direct complementation of aRhizobium nodD-mutant
Clones from aFrankia At4 gene bank were pooled into groups and mass conjugated into anodD mutant ofRhizobium leguminosarum bv.viciae by triparental matings. When peas were inoculated with the pooled transconjugan...
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Article
Modulation of the growth and morphology of a human nasopharyngeal carcinoma cell line by growth factors
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Chapter
The Yu-cheng Rice Oil Poisoning Incident
As human beings, we hope to learn from past experiences and not repeat our mistakes. Unfortunately, 11 years after the Japanese Yusho incident (see Chapter 19), a similar tragedy happened in Taiwan in 1979. A ...
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Article
Production of virus resistant and insect tolerant transgenic tobacco plants
The cucumber mosaic virus coat protein (CMV-CP) gene and a modified Bacillus thuringiensis δ-endotoxin (Bt toxin) gene were cloned into plant expression vector pE3. Tobacco (Nicotiana tabacum cv. G28) leaf discs ...
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Article
Subunit interaction enhances enzyme activity and stability of sweet potato cytosolic Cu/Zn-superoxide dismutase purified by a His-tagged recombinant protein method
The coding region of copper/zinc-superoxide dismutase (Cu/Zn-SOD) cDNA from sweet potato, Ipomoea batatas (L.) Lam. cv. Tainong 57, was introduced into an expression vector, pET-20b(+). The Cu/Zn-SOD purified by ...
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Article
Transposon mutagenesis and cloning of the genes encoding the enzymes of fengycin biosynthesis inBacillus subtilis
A total of 20Bacillus subtilis F29-3 mutants defective in fengycin biosynthesis was obtained by Tn917 mutagenesis. Cloning and map** results showed that the transposon in these mutants was inserted in eleven di...
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Article
Characterization of the replicon of plasmid pSW500 ofErwinia stewartii
A 1.6-kb DNA region required for the replication of pSW500 fromErwinia stewartii SW2 has been identified. DNA sequencing analysis revealed that this DNA fragment consists of a DnaA box, seven 16-bp direct repeats...
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Article
Transgenic sweet pepper plants fromAgrobacterium mediated transformation
Fertile transgenic sweet pepper (Capsicum annuum var. grossum) plants were regenerated at relatively high rate from various explants that were cocultivated withAgrobacterium tumefaciens strain GV3111-SE harbourin...
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Understanding Genetic Control of Freezing Resistance Using Potato Species as a Model System
Freezing stress resistance is generally regarded as a complex trait with multigenic inheritance (Marshall, 1982; Stushnoff et al., 1984; Blum, 1988). However, there is no consensus on the mode of gene action. For...
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Article
Abstracts of papers presented at the 81st annual meeting of The Potato Association of America Charlottetown, P.E.I., Canada August 3 – 7, 1997
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Chapter
Antitumor Activity of Yunnanmycin
Nucleoside salvage in nucleotide biosynthesis is a subject of great concern in cancer chemotherapy. Antimetabolites that currently used for cancer treatment inhibit de nono pathways of nucleotide synthesis fail t...
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Article
Candida Rugosa Lipase Catalyzed Esterification of Racemic Ibuprofen with Butanol: Racemization of R-Ibuprofen and Chemical Hydrolysis of S-Ester Formed
The chemical treatment of products obtained from Candida rugosa lipase catalyzed enantioselective esterification of racemic ibuprofen with n-butanol in isooctane was studied. After a complete separation of the...
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Article
Gibberellin-responding and non-responding dwarf mutants in foxtail millet
The sensitivity of foxtail millet (Setaria italica Beauv.) dwarf mutants to GA was studied. Seedlings of dwarf mutants, Aininghuang (ANH) and CH84113 were treated with GAs (GA1, GA4, GA9, GA19 and GA20) using ...
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Article
Expression of nonacclimated freezing tolerance and cold acclimation capacity in somatic hybrids between hardy wild Solanum species and cultivated potatoes
The expression of freezing tolerance was characterized in interspecific somatic hybrids between S. tuberosum (tbr) and two cold-hardy wild species, S. brevidens (brd) and S. commersonii (cmm). The nonacclimate...
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Article
Ri-mediated transformation of Artemisia annua with a recombinant farnesyl diphosphate synthase gene for artemisinin production
A transgenic system was developed for Artemisia annua L. via Agrobacterium rhizogenes-mediated transformation. Using this system a cDNA encoding farnesyl diphosphate synthase (FDS) placed under a CaMV 35S promote...
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Article
Improvement of plant regeneration from long-term cultured calluses of Taipei 309, a model rice variety in in vitro studies
An efficient protocol was developed for regeneration of plants from long-term cultured calluses, which originated from mature seeds of a model rice variety Taipei 309 and were maintained by subculture for at l...
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Culture of skeletal muscle cells from giant panda
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Article
Chemical composition and potential for utilization of the marine alga Rhizoclonium sp.
This paper reports on the feasibility of utilizing the abundant marine alga Rhizoclonium as a substitute for wood fiber, based on studies on its morphology and chemical composition. The alga appears as wood fiber...
