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Chaoyong **a
Biotechnology
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Article
Open AccessProbiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens
Baicalin and probiotic cocktails are promising feed additives with broad application prospects. While probiotic cocktails are known to enhance intestinal health, the potential synergistic impact of combining b...
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Article
Decoder-seq enhances mRNA capture efficiency in spatial RNA sequencing
Spatial transcriptomics technologies with high resolution often lack high sensitivity in mRNA detection. Here we report a dendrimeric DNA coordinate barcoding design for spatial RNA sequencing (Decoder-seq), w...
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Article
Recent progress in co-detection of single-cell transcripts and proteins
Cellular heterogeneity is a universal property of living systems, and the interrogation of single cells facilitates in-depth understanding of distinct cellular states and functions in various biological proces...
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Living Reference Work Entry In depth
Aptamer Molecular Evolution for Liquid Biopsy
Liquid biopsy can provide comprehensive and real-time physiological and pathological information in a noninvasive and longitudinal monitoring manner, facilitating precision medicine practice and clinical inves...
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Reference Work Entry In depth
Aptamer Molecular Evolution for Liquid Biopsy
Liquid biopsy can provide comprehensive and real-time physiological and pathological information in a noninvasive and longitudinal monitoring manner, facilitating precision medicine practice and clinical inves...
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Article
Systemic delivery of microRNA for treatment of brain ischemia
Brain ischemia is the second leading cause of death and the third leading cause of disability in the world. Systemic delivery of microRNA, a class of molecules that regulate the expression of cellular proteins...
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Article
Efficient long fragment editing technique enables large-scale and scarless bacterial genome engineering
Bacteria are versatile living systems that enhance our understanding of nature and enable biosynthesis of valuable chemicals. Long fragment editing techniques are of great importance for accelerating bacterial...
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Article
Oligolayered Ti3C2Tx MXene towards high performance lithium/sodium storage
2D MXenes are highly attractive for achieving ultrafast and stable lithium/sodium storage due to their good electric conductivity and abundant redox active sites. While, effective strategies for scalable prepa...
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Article
Open AccessReversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli
Co-expression of two distinct guide RNAs (gRNAs) has been used to facilitate the application of CRISPR/Cas9 system in fields such as large genomic deletion. The paired gRNAs are often placed adjacently in the ...
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Article
CRISPR-Cas9-assisted native end-joining editing offers a simple strategy for efficient genetic engineering in Escherichia coli
Unlike eukaryotes, prokaryotes are less proficient in homologous recombination (HR) and non-homologous end-joining (NHEJ). All existing genomic editing methods for Escherichia coli (E. coli) rely on exogenous HR ...
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Chapter
The Clinical Application of Aptamers: Future Challenges and Prospects
This final chapter attempts to search for reasons to explain why so little progress has been made in the practical clinical application of aptamers and propose potential solutions to the problem. The advantage...
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Book
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Chapter
Molecular Beacons for Detection of Single-Nucleotide Polymorphisms
Molecular beacons have been employed for the detection of single-nucleotide polymorphisms (SNPs) and allele discrimination because of their excellent selectivity in distinguishing single-nucleotide mutation. M...
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Chapter
Application of Molecular Beacons in Real-Time PCR
Real-time PCR or quantitative PCR (QPCR) is a powerful technique that allows measurement of PCR product while the amplification reaction proceeds. It incorporates the fluorescent element into conventional PCR ...
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Protocol
Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR
Agarose emulsion droplet microfluidic technology for single copy emulsion PCR (ePCR) is a suitable technique for the detection of single copy DNA molecules. It improves the traditional ePCR by employing agaros...
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Chapter
Molecular Aptamer Beacons
In this chapter, we present a special class of nucleic acid probes, called molecular aptamer beacons, which combine the specific recognition of aptamers and the signal transduction of molecular beacons. By usi...
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Chapter
Engineering Molecular Beacons for Advanced Applications
As nucleic acid-based probes, molecular beacons (MBs) have shown promising applications in genetic screening, biosensor development, and single-nucleotide polymorphism detection. The chemical nature of nucleic...
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Chapter
Functional Nucleic Acids for DNA Nanotechnology
Today, DNA emerged as a fundamental and intelligent molecule to assist construction and functionalization of nanodevices in the field of nanotechnology. Besides the powerful base-pair molecular recognition pro...
