26 Result(s)
within
C. BENITO
Plant Biochemistry
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Article
Candidate gene identification of an aluminum-activated organic acid transporter gene at the Alt4 locus for aluminum tolerance in rye (Secale cereale L.)
Among cereal crops, rye is one of the most tolerant species to aluminum. A candidate gene approach was used to determine the likely molecular identity of an Al tolerance locus (Alt4). Using PCR primers designed f...
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Article
A new aluminum tolerance gene located on rye chromosome arm 7RS
Rye has one of the most efficient groups of genes for aluminum tolerance (Alt) among cultivated species of Triticeae. This tolerance is controlled by, at least, three independent and dominant loci (Alt1, Alt2, an...
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Article
The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin
The potential of bulk analyses of RAPD and ISSR-PCR markers for fingerprinting purposes was evaluated using ten RAPD and ten ISSR primers. The phylogenetic relationships of 16 barley cultivars from different c...
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Article
Is the Mnr locus of Triticeae species the same as the Ndh and Dia loci?
The menadione reductase (MNR), the nicotinamide adenine dinucleotide dehydrogenase (NDH) and diaphorase (DIA) isozymes were studied in the allohexaploid Triticum aestivum cv ”Chinese Spring” and in five diploid T...
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Article
Molecular markers linked to the aluminium tolerance gene Alt1 in rye (Secale cereale L.)
Rye has one of the most efficient group of genes for aluminium (Al) tolerance among cultivated species of Triticeae. This tolerance is controlled by at least two independent and dominant loci (Alt1 and Alt3) loc...
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Article
Chromosomal location of PCR fragments as a source of DNA markers linked to aluminium tolerance genes in rye
To identify and locate rye DNA sequences homologous to three wheat c-DNAs (wali1, wali2 and wali5) whose expression is induced by aluminium (Al) stress, we designed three pairs of specific primers. They were used...
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Article
Genetic control of aluminium tolerance in rye (Secale cereale L.)
Aluminium (Al) tolerance in roots of two cultivars (“Ailés” and “JNK”) and two inbred lines (“Riodeva” and “Pool”) of rye was studied using intact roots immersed in a nutrient solution at a controlled pH and ...
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Article
Sex influence on recombination frequency in Secale cereale L.
The variation in recombination frequency (rf) is important to plant breeders since their major objective is to obtain favorable recombinants of linked genes. One source of variation in rf is sex. Sex differenc...
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Article
A map of rye chromosome 4R with cytological and isozyme markers
The progeny of two crosses between a structural heterozygote for a reciprocal translocation (4RL/5RL) and a homozygote for the standard chromosome arrangement and of four crosses between standard chromosome ho...
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Article
NADH dehydrogenase: a new molecular marker for homoeology group 4 in Triticeae. A map of the 4RS chromosome arm in rye
Structural gene loci encoding the monomeric isozymes nicotin adenin dinucleotide dehydrogenase (NADH dehydrogenase or NDH) have been located on the 4AL, 4Bα, and 4DS chromosome arms of Triticum aestivum cv “Chine...
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Article
Biochemical evidence of a translocation between 6 RL/7 RL chromosome arms in rye (Secale cereale L.). A genetic map of 6R chromosome
The segregation of different isozymic loci was investigated in backcrosses and F2s in rye. The leucin aminopeptidase-1 (Lap-1), Aconitase-1 (Aco-1), Esterase-6 (Est-6), Esterase-8 (Est-8), and Endopeptidase-1 (Ep...
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Article
A map of rye chromosome 2R using isozyme and morphological markers
The segregation of isozymic loci for leaf peroxidases (L2Per) has been investigated in backcrosses and F2 offspring of rye lines having purple seeds (Ps) and monstrosum ears (mo). The Ps, L2Per-3b, mo, and L2Per-...
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Article
Linkage and cytogenetic maps of genes controlling endosperm storage proteins and isozymes in rye (Secale cereale L.)
An F1 plant fromSecale cereale ssp.ancestrale xtelocentric substitution lines3R of the cultivated rye “Petkus spring” was used as female in a cross with the inbred line Riodeva (I28), which has the standard chrom...
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Article
Chromosomal location of adenylate kinase isozymes in Triticeae species
One system of monomeric adenylate kinase isozymes, designated ADK, was observed in Triticum aestivum and in five diploid Triticeae species. The gene loci Adk-a, Adk-b and Adk-d were located on 7AL, 7BL and 7DL Tr...
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Article
Association of four isozyme loci with a reciprocal translocation between 1R/4R chromosomes in cultivated rye (Secale cereale L.)
The progeny of four crosses between a structural heterozygote for a reciprocal translocation and a homozygote for the standard chromosome arrangement were analyzed in rye (Secale cereale L. cv “Ailés”) for the el...
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Article
Genetics of rye phosphatases: evidence of a duplication
Genetic analyses were conducted on alkaline phosphatases of the endosperm of dry kernels and leaf acid phosphatases in four open pollinated and one inbred line of cultivated rye (Secale cereale L.). A total of se...
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Article
The peroxidase isozymes of the wheat kernel: tissue and substrate specificity and their chromosomal location
Peroxidase isozymes were studied in the Triticum aestivum L. kernel and in nullisomic-tetrasomic and ditelocentric combinations of ‘Chinese Spring’ wheat. Analyses were carried out on different parts of dry kerne...
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Article
Location of genes coding isozyme markers on Aegilops umbellulata chromosomes adds data on homoeology among Triticeae chromosomes
Zymogram analysis was used to identify the Aegilops umbellulata chromosomes that carry the structural genes for particular isozymes. Wheat, Aegilops and wheat-Aegilops hybrid derivative lines (which contained ide...
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Article
Biochemical evidence of homoeology between Triticum aestivum and Agropyron intermedium chromosomes
The alcohol dehydrogenase (ADH), phosphoglucose mutase (PGM), glucosephosphate isomerase (GPI), glutamic oxaloacetic transaminase (GOT), malate dehydrogenase (MDH), leaf esterases (ESTL), leaf acid (ACPH) and ...
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Article
Esterase isozymes in rye — characterization, genetic control and chromosomal location
The zymogram phenotypes that ‘Chinese Spring’-‘Imperial’, ‘Holdfast’-‘King II’ and ‘Kharkov’-‘Dakold’ wheat-rye addition lines presented for esterase isozymes were determined using polyacrylamide gel ectrophor...
