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Article
One-step Purification and Immobilization in Cellulose of the GroEL Apical Domain Fused to a Carbohydrate-binding Module and its use in Protein Refolding
The apical domain of the chaperonin, GroEL, fused to the carbohydrate binding module type II, CBDCex, of Cellulomonas fimi, was expressed in Escherichia coli. The recombinant protein, soluble or from inclusion bo...
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Article
Efficient Transformation of Cellulomonas flavigena by Electroporation and Conjugation with Bacillus thuringiensis
The conjugative self-transmissible plasmid pHT73, harbored in Bacillus thuringiensis var. kurstaki, was demonstrated to be transferred to Cellulomonas flavigena, a cellulolytic bacterium. Both conjugation and tra...
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Article
Carboxin-Resistant Mutant of Ustilago maydis Is Impaired in Its Pathogenicity for Zea mays
We analyzed the pathogenicity of chitin synthetase (chs) disruptants of Ustilago maydis obtained with the carboxin-resistant or the hygromycin-resistant cassettes. We found that only chitin synthetase (chs) muta...