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Article
Lambda red-mediated synthesis of plasmid linear multimers in Escherichia coli K12
Expression of the red + and gam + genes of bacteriophage λ in plasmids cloned in Escherichia coli wild-type cells leads to plasmid linear multimer (PLM) formation. In mutants that lack exonuclease I (sbcB sbcC), ...
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Article
Plasmidic recombination in Escherichia coliK-12: The role of recF gene function
Interplasmidic and intraplasmidic recombination proficiencies were determined in E. coli bacterial strains carrying rec mutations. Our results defined the role of recF gene function, recB, recC, and sbcB gene pro...
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Article
Interplasmidic and intraplasmidic recombination in Escherichia coli K-12
The construction of plasmids which facilitate the study of interplasmidic and intraplasmidic recombination is described. In this system, a single recombination event between two mutated Ter genes on separate plas...