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    Article

    Intracellular directed evolution of proteins from combinatorial libraries based on conditional phage replication

    This protocol describes a phagemid-based intracellular evolution approach to generate and select for proteins with improved biological characteristics.

    Andreas K Brödel, Alfonso Jaramillo, Mark Isalan in Nature Protocols (2017)

  2. Article

    Open Access

    Automated design of bacterial genome sequences

    Organisms have evolved ways of regulating transcription to better adapt to varying environments. Could the current functional genomics data and models support the possibility of engineering a genome with compl...

    Javier Carrera, Alfonso Jaramillo in BMC Systems Biology (2013)

  3. Article

    Open Access

    Robust dynamical pattern formation from a multifunctional minimal genetic circuit

    A practical problem during the analysis of natural networks is their complexity, thus the use of synthetic circuits would allow to unveil the natural mechanisms of operation. Autocatalytic gene regulatory netw...

    Guillermo Rodrigo, Javier Carrera, Santiago F Elena in BMC Systems Biology (2010)

  4. Article

    Open Access

    Computational design of proteins with new functions

    Alfonso Jaramillo, Pablo Tortosa, Guillermo Rodrigo, Maria Suarez in BMC Systems Biology (2007)

  5. Article

    Open Access

    ECOLITASTER: cellular biosensor

    Guillermo Rodrigo, Javier Carreral, Alfonso Jaramillo in BMC Systems Biology (2007)