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Bright and stable monomeric green fluorescent protein derived from StayGold

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  1. Article

    Open Access

    Structure-based rational design of an enhanced fluorogen-activating protein for fluorogens based on GFP chromophore

    “Fluorescence-Activating and absorption-Shifting Tag” (FAST) is a well-studied fluorogen-activating protein with high brightness and low size, able to activate a wide range of fluorogens. This makes FAST a pro...

    Marina V. Goncharuk, Nadezhda S. Baleeva, Dmitry E. Nolde in Communications Biology (2022)

  2. Article

    Open Access

    DiB-splits: nature-guided design of a novel fluorescent labeling split system

    Fluorogen-activating proteins (FAPs) are innovative fluorescent probes combining advantages of genetically-encoded proteins such as green fluorescent protein and externally added fluorogens that allow for high...

    Nina G. Bozhanova, Alexey S. Gavrikov, Alexander S. Mishin in Scientific Reports (2020)