12 Result(s)
within
Akio Kato
Life Sciences
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Article
Haploid plants carrying a sodium azide-induced mutation (fdr1) produce fertile pollen grains due to first division restitution (FDR) in maize (Zea mays L.)
We induced a fdr1 mutation in maize which makes haploid plants male fertile due to first division restitution; the optimum sodium azide treatment on maize ke...
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Article
Phenotypic and gene expression analyses of a ploidy series of maize inbred Oh43
Polyploidization has repeatedly occurred during plant evolution. Although autopolyploidy is the best model to characterize the polyploidization effects in a highly controlled manner, there are limited studies ...
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Protocol
Chromosome Painting for Plant Biotechnology
Fluorescence in situ hybridization (FISH) is an invaluable tool for chromosome analysis and engineering. The ability to visually localize endogenous genes, transposable elements, transgenes, naturally occurrin...
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Article
The effect of calnexin deletion on the expression level of binding protein (BiP) under heat stress conditions in Saccharomyces cerevisiae
In order to investigate the effect of calnexin deletion on the induction of the main ER molecular chaperone BiP, we cultured the wild-type and calnexin-disrupted Saccharomyces cerevisiae strains under normal and ...
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Article
Agrobacterium-mediated transformation of maize (Zea mays) with Cre-lox site specific recombination cassettes in BIBAC vectors
The Cre/loxP site-specific recombination system has been applied in various plant species including maize (Zea mays) for marker gene removal, gene targeting, and functional genomics. A BIBAC vector system was ada...
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Article
The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions
We cultured calnexin-disrupted and wild-type Saccharomyces cerevisiae strains under conditions of heat stress. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast un...
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Article
Distinct chromosomal distributions of highly repetitive sequences in maize
The majority of genomic DNA in most plant species is made up of repetitive elements including satellites and retrotransposons. The maize genome is intermediate in size and abundance of repetitive elements betw...
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Article
Genetic variation for the response to ploidy change in Zea mays L.
Polyploidization is an important process in the evolutionary history of most eukaryotic species. It oftentimes causes large-scale genomic reorganizations and is accompanied by a wide variety of phenotypic alte...
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Article
A test for ectopic exchange catalyzed by Cre recombinase in maize
A maize line expressing Cre recombinase as well as the recipient line without the transgene were assayed for evidence of ectopic recombination within the maize genome. Such a test is valuable for understanding...
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Article
Sequences associated with A chromosome centromeres are present throughout the maize B chromosome
Maize chromosome spreads containing the supernumerary B chromosome were hybridized with probes from various repetitive elements including CentC, CRM, and CentA, which have been localized to centromeric regions...
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Article
Effects of stand density on the productivity ofCryptomeria japonica male flowers
We have studied male flower production and relative illumination in clonal sugi (Cryptomeria japonica) forests planted at densities of 1,500/ha, 3,000/ha, and 5,000/ha, to clarify the effects of forest management...
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Article
Induced single fertilization in maize
Bicellular pollen with one vegetative nucleus and one diploid arrested generative cell (”monospermic” pollen) was induced by trifluralin treatment of diploid maize plants at 7–9 days before flowering. The arr...
