260 Result(s)
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Article
Somatic hybridization between Citrus reticulata and Citropsis gabunensis through electrofusion
Protoplasts from embryogenic calli of Citrus reticulata Blanco cv. Ponkan and Citropsis gabunensis (Engl.) Swing. & M. Kell (Cabon Cherry Orange), were isolated and fused using electric current. Maximum fusion fr...
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Article
Isolation and culture of daylily mesophyll protoplasts
Mesophyll protoplasts were isolated from leaf tissues of a diploid daylily (Hemerocallisx‘Red Magic’) by enzymatic digestion with a solution containing 0.5% Pectolyase Y-23, 0.1% Cellulase R-10, 0.1% Driselase, 0...
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Protocol
Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation
Embryonic stem (ES) cells, the undifferentiated cells of early embryos are established as permanent lines (1,2) and are characterized by their self-renewal capacity and the ability to retain their developmental c...
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Article
LATS1 tumour suppressor affects cytokinesis by inhibiting LIMK1
LATS (large tumour suppressor) is a family of conserved tumour suppressors identified in Drosophila and mammals. Here we show that human LATS1 binds to LIMK1 in vitro and in vivo and colocalizes with LIMK1 at the...
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Protocol
Hematopoietic Development of Human Embryonic Stem Cells in Culture
The isolation of embryonic stem (ES) cells from human preimplantation blastocysts creates an exciting new starting point to analyze the earliest stages of human blood development. This chapter describes two me...
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Protocol
Radionuclide-Peptide Nucleic Acid in Diagnosis and Treatment of Pancreatic Cancer
Cancer of the exocrine pancreas will attack an estimated 31,000 Americans in 2004, causing an estimated 30,000 deaths (1). Cancer of the exocrine pancreas (hereafter simply referred to as pancreatic cancer) occur...
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Protocol
Hematopoietic Development of Human Embryonic Stem Cells in Culture
The successful isolation and characterization of human embryonic stem cells (hESCs) provides a powerful tool to study the cellular and genetic mechanisms that mediate cell-fate decisions toward distinct develo...
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Article
Short-term treatment with retinoic acid prevents spontaneous differentiation of murine embryonic stem cells
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Article
The block of intracellular calcium release affects the pollen tube development of Picea wilsonii by changing the deposition of cell wall components
Two potent drugs, neomycin and TMB-8, which can block intracellular calcium release, were used to investigate their influence on pollen tube growth and cell wall deposition in Picea wilsonii. Apart from inhibitin...
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Article
Phosphorylation of ATM by Cdk5 mediates DNA damage signalling and regulates neuronal death
CDK5 kinase regulates the neuronal stress response, whereas the DNA damage response kinase ATM induces apoptosis in postmitotic neurons. Now, CDK5 activated by DNA damage is shown to directly activate ATM to i...
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Article
Ubiquitin B: an essential mediator of trichostatin A-induced tumor-selective killing in human cancer cells
Although histone deacetylase inhibitors (HDACis) are emerging as a new class of anticancer agents, the mechanism of tumor-selective killing by HDACi is not well understood. We used suppression of mortality by ...
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Article
Activation of p38 MAPK Pathway by Hepatitis C Virus E2 in Cells Transiently Expressing DC-SIGN
Dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a cellular receptor for hepatitis C virus for the binding of viral envelope glycoprotein E2. Interaction of DC-SIGN w...
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A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy
The main aims of this study were to determine the effects of GH gene abuse/misuse in normal animals and to discover genes that could be used as candidate biomarkers for the detection of GH gene therapy abuse/m...
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Differentiation of cryopreserved human umbilical cord blood-derived stromal cells into cells with an oligodendrocyte phenotype
In this study, we examined the phenotypic characteristics of human umbilical cord blood-derived mesenchymal stromal cells (UCB-derived MSCs) differentiated along an oligodendrocyte pathway. We induced human UC...
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Article
Schwann-like cells can be induction from human nestin-positive amniotic fluid mesenchymal stem cells
We examined the morphological, phenotypic, and functional characteristics of human amniotic fluid mesenchymal stem cells (AF-MSCs) differentiated towards a Schwann cell lineage. Initially, we induced human AF-...
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Article
Open AccessMacrophage colony-stimulating factor and its receptor signaling augment glycated albumin-induced retinal microglial inflammation in vitro
Microglial activation and the proinflammatory response are controlled by a complex regulatory network. Among the various candidates, macrophage colony-stimulating factor (M-CSF) is considered an important cyto...
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Article
Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals
Although myocyte cell transplantation studies have suggested a promising therapeutic potential for myocardial infarction, a major obstacle to the development of clinical therapies for myocardial repair is the ...
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Article
A tyrosine decarboxylase catalyzes the initial reaction of the salidroside biosynthesis pathway in Rhodiola sachalinensis
Salidroside, the 8-O-β-d-glucoside of tyrosol, is the main bioactive component of Rhodiola species and is found mainly in the plant roots. It is well known that glucosylation of tyrosol is the final step in the b...
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Article
PKH26 as a fluorescent label for live human umbilical mesenchymal stem cells
To determine whether PKH26 labeling affects the morphologies, phenotypes, proliferation, and secretion abilities of human umbilical mesenchymal stromal cells (HUMSCs) were investigated. Isolated HUMSCs were la...
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Article
In vitro differentiation of rat embryonic stem cells into functional cardiomyocytes
The recent breakthrough in the generation of rat embryonic stem cells (rESCs) opens the door to application of gene targeting to create models for the study of human diseases. In addition, the in vitro differenti...
