186 Result(s)
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Article
Oxygen regulation of uricase and sucrose synthase synthesis in soybean callus tissue is exerted at the mRNA level
The effect of lowering oxygen concentration on the expression of nodulin genes in soybean callus tissue devoid of the microsymbiont has been examined. Poly(A)+ RNA was isolated from tissue cultivated in 4% oxygen...
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Article
Characterization of a Gy4 glycinin gene from soybean Glycine max cv. Forrest
The glycinin gene family encoding the glycinin subunits in soybean plants is composed of at least five gene members. A genomic clone λS312 containing the Gy4 gene from a genomic library of cv. Forrest was isolate...
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Article
Isolation and characterization of a cDNA from flowers of Cynara cardunculus encoding cyprosin (an aspartic proteinase) and its use to study the organ-specific expression of cyprosin
Poly(A)+ RNA isolated from flower buds of Cynara cardunculus has been used to prepare a cDNA library. Screening of the cDNA after expression of cloned DNA with antibodies raised against the large subunit of cypro...
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Protocol
Monoclonal Antibodies to Cytochromes P450
Monoclonal antibodies (MAbs) are produced by the “hybridoma” technology developed by Kohler and Milstem in 1975 (1), which is a major and defining achievement of modern biology (2). The hybridoma methodology prod...
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Article
Olfactory Responses of Ips duplicatus from Inner Mongolia, China to Nonhost Leaf and Bark Volatiles
Leaf and bark volatiles from nonhost angiosperm trees were tested on Ips duplicatus by gas chromatographic–electroantennographic detection (GC-EAD) and by pheromone-baited traps in Sweden and Inner Mongolia, C...
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Chapter and Conference Paper
Continuous Production of Butanol by Clostridium acetobutylicum Immobilized in a Fibrous Bed Bioreactor
We explored the influence of dilution rate and pH in continuous cultures of Clostridium acetobutylicum. A 200-mL fibrous bed bioreactor was used to produce high cell density and butyrate concentrations at pH 5.4 ...
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Article
Dynamic changes in transcription factor complexes during erythroid differentiation revealed by quantitative proteomics
During erythroid differentiation, β-globin gene expression is regulated by the locus control region (LCR). The transcription factor NF-E2p18/MafK binds within this region and is essential for β-globin expressi...
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Chapter and Conference Paper
A Hollow-Fiber Membrane Extraction Process for Recovery and Separation of Lactic Acid from Aqueous Solution
An energy-efficient hollow-fiber membrane extraction process was successfully developed to separate and recover lactic acid produced in fermentation. Although many fermentation processes have been developed fo...
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Article
Electrophysiological and Behavioral Responses of Ips duplicatus to Aggregation Pheromone in Inner Mongolia, China: Amitinol as a Potential Pheromone Component
Volatiles from Ips duplicatus male hindgut extracts and aeration samples of spruce logs colonized by the virgin males were analyzed by coupled gas chromatography–mass spectrometry (GC-MS), coupled gas chromatogra...
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Protocol
Hematopoietic Development of Human Embryonic Stem Cells in Culture
The successful isolation and characterization of human embryonic stem cells (hESCs) provides a powerful tool to study the cellular and genetic mechanisms that mediate cell-fate decisions toward distinct develo...
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Article
Reduced expression of GSTM2 and increased oxidative stress in spontaneously hypertensive rat
Human essential hypertension is a complex polygenic trait with underlying genetic components that remain unknown. The spontaneously hypertensive rat (SHR) is a well-characterized experimental model for essenti...
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Article
Effects of cassava starch hydrolysate on cell growth and lipid accumulation of the heterotrophic microalgae Chlorella protothecoides
Heterotrophic fermentation of microalgae has been shown to accumulate high amounts of microalgal lipids, which are regarded as one of the most promising feedstocks for sustainable biodiesel production. To incr...
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Article
Potential adenovirus-mediated gene therapy of glioma cancer
Malignant gliomas are typically characterized by rapid cell proliferation and a marked propensity to invade and damage surrounding tissues. They are the main brain tumors notoriously resistant to currently ava...
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Article
Ubiquitin B: an essential mediator of trichostatin A-induced tumor-selective killing in human cancer cells
Although histone deacetylase inhibitors (HDACis) are emerging as a new class of anticancer agents, the mechanism of tumor-selective killing by HDACi is not well understood. We used suppression of mortality by ...
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Article
Activation of p38 MAPK Pathway by Hepatitis C Virus E2 in Cells Transiently Expressing DC-SIGN
Dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a cellular receptor for hepatitis C virus for the binding of viral envelope glycoprotein E2. Interaction of DC-SIGN w...
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Article
A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy
The main aims of this study were to determine the effects of GH gene abuse/misuse in normal animals and to discover genes that could be used as candidate biomarkers for the detection of GH gene therapy abuse/m...
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Article
Validation of reference genes of grass carp Ctenopharyngodon idellus for the normalization of quantitative real-time PCR
Expression of four reference genes of grass carp, including β-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S rRNA (18S) and elongation factor-1 alpha (EF1α), was studied in tissues of norm...
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Article
Isolation and characterization of an AHL lactonase gene from Bacillus amyloliquefaciens
An AHL lactonase gene (aiiA) was PCR amplified from the genomic DNA of Bacillus amyloliquefaciens, with the intact open reading frame of 753 base pair. The gene shares high identity to its homologues present in d...
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Article
Down-regulation of IGF-1R expression inhibits growth and enhances chemosensitivity of endometrial carcinoma in vitro
The type-1 insulin-like growth factor receptor (IGF-1R) is over-expressed by endometrial carcinoma, level of IGF-1R has been correlated with tumor progression, and high IGF-1R expression has been found to be a...
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Article
Transgenic expression of TaMYB2A confers enhanced tolerance to multiple abiotic stresses in Arabidopsis
Osmotic stresses such as drought, salinity, and cold are major environmental factors that limit agricultural productivity. Transcription factors play essential roles in abiotic stress signaling in plants. Three T...
