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Article
Study of the degradation of a multidrug transporter using a non-radioactive pulse chase method
Proteins are constantly synthesized and degraded in living cells during their growth and division, often in response to metabolic and environmental conditions. The synthesis and breakdown of proteins under dif...
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Article
Cysteine residue is not essential for CPM protein thermal-stability assay
A popular thermal-stability assay developed especially for the study of membrane proteins uses a thiol-specific probe, 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM). The fluorescence emission of ...
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Article
Structural characterization and antioxidant properties of polysaccharides from two Schisandra fruits
Different polysaccharides (NPS1-1 and BPS1-1) were obtained from Schisandra sphenanthera and Schisandra chinensis fruits by hot-water extraction, DEAE-52 ion-exchange column chromatography and Sephadex G-150 gel ...
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Article
Physicochemical characterization and antioxidant activity of a polysaccharide isolated from oolong tea
Oolong tea polysaccharide (OTPS) is a kind of water-soluble polysaccharide isolated from oolong tea. Its molecular weight, composition, and structure were analyzed by laser light scattering analysis, chemical ...