326 Result(s)
within
Biotechnology
**ao-**a Jiang
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Article
Allotetraploid hybrids between citrus and seven related genera produced by somatic hybridization
We have developed an efficient protoplast-fusion method to produce somatic hybrid allopolyploid plants that combine Citrus with seven related genera, including four that are sexually incompatible. In this paper w...
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Article
Open AccessEffective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer.
Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limi...
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Chapter and Conference Paper
Land degradation and blown-sand disaster in China
China is a country with severe land degradation and blown sand disasters. The arid and semi-arid regions, in which land desiccation, vegetation degeneration, wind erosion, sandification, Gobi-pebblization and ...
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Article
A novel method to monitor the expression of microRNAs
The microRNAs (miRNAs) are an extensive class of small noncoding RNAs (18–25 nucleotides) with important roles in the regulation of gene expression. Although a large number of miRNAs have been identified in a ...
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Article
Efficient induction of ginsenoside biosynthesis and alteration of ginsenoside heterogeneity in cell cultures of Panax notoginseng by using chemically synthesized 2-hydroxyethyl jasmonate
Chemically synthesized 2-hydroxyethyl jasmonate (HEJA) was for the first time employed to induce the ginsenoside biosynthesis and to manipulate the product heterogeneity in plant cell cultures. The dose respon...
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Article
In silico pharmacogenetics of warfarin metabolism
Pharmacogenetic approaches can be instrumental for predicting individual differences in response to a therapeutic intervention. Here we used a recently developed murine haplotype-based computational method to ...
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Article
Protoplast Fusion between Geotrichum candidium and Phanerochaete chrysosporium to Produce Fusants for Corn Stover Fermentation
Lignin impedes the digestion of corn stover when used as an animal feed. Phanerochaete chrysosporium is an efficient lignindegrader. Geotrichum candidum can be used to produce single-cell protein. In this study, ...
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Article
Remediation of pentachlorophenol-contaminated soil by composting with immobilized Phanerochaete chrysosporium
To reduce and eliminate the hazards of pentachlorophenol (PCP) to the soil, the method of inoculating free and immobilized white rot fungi, Phanerochaete chrysosporium to PCP-polluted soils was investigated. Thre...
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Article
Bioconversion of ethyl 4-chloro-3-oxobutanoate by permeabilized fresh brewer’s yeast cells in the presence of allyl bromide
Ethyl(R)-4-chloro-3-hydroxybutanoate ((R)-CHBE) are obtained by cetyltrimetylammonium bromide (CTAB) permeabilized fresh brewer’s yeast whole cells bioconversion of ethyl 4-chloro-3-oxobutanoate (COBE ) in the...
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Article
Expression of open reading frames in silkworm pupal cDNA library
A cDNA library containing 2409 singletons was constructed from whole silkworm pupae (Bombyx mori) In addition, the types of genes overexpressed in pupa were analyzed. These genes contained 79 types of proteins wi...
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Article
Enhancement of growth and polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense by the addition of putrescine
Putrescine at 0.6 mM stimulated protocorm-like body growth and polysaccharide synthesis in suspension cultures of Dendrobium huoshanense. The specific growth rate of protocorm-like body increased from 0.047 to 0....
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Article
A trypsin inhibitor from Cassia obtusifolia seeds: isolation, characterization and activity against Pieris rapae
A trypsin inhibitor was isolated from Cassia obtusifolia by ammonium sulfate precipitation, Sepharose 4B-trypsin affinity and Sephadex G-75 chromatography. The inhibitor consisted of a single polypeptide chain wi...
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Article
Open AccessQuantum dot imaging for embryonic stem cells
Semiconductor quantum dots (QDs) hold increasing potential for cellular imaging both in vitro and in vivo. In this report, we aimed to evaluate in vivo multiplex imaging of mouse embryonic stem (ES) cells labeled...
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Article
Inhibition Kinetics of Cabbage Butterfly (Pieris rapae L.) Larvae Phenoloxidase Activity by 3-Hydroxy-4-Methoxybenzaldehyde Thiosemicarbazone
Phenoloxidase (PO) is a key enzyme in insect development, responsible for catalyzing the hydroxylation of tyrosine into o-diphenols and the oxidation of o-diphenols into o-quinones. In the present study, the kine...
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Article
Biodegradation of Ethametsulfuron-Methyl by Pseudomonas sp. SW4 Isolated from Contaminated Soil
A soil bacterium SW4, capable of degrading the sulfonylurea herbicide ethametsulfuron-methyl (ESM), was isolated from the bottom soil of a herbicide factory. Based on physiological characteristics, biochemical...
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Article
Cloning, expression, and pharmacological activity of BmK AS, an active peptide from scorpion Buthus martensii Karsch
BmK AS is a β long-chain scorpion peptide from the venom of Buthus martensii Karsch (BmK). It was efficiently expressed as a soluble and functional peptide in Escherichia coli, and purified by metal chelating chr...
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Article
Small interfering RNA knockdown of mini-TyrRS and mini-TrpRS effects angiogenesis in human umbilical vein endothelial cells in hypoxic culture
Aim We studied the role of mini-TyrRS and mini-TrpRS in angiogenesis by using small interfering RNA-mediated mini-TyrRS/mini-TrpRS knockout in hypoxic culture of human umbilical vein endo...
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Article
Salinicoccus salitudinis sp. nov., a new moderately halophilic bacterium isolated from a saline soil sample
A novel pale-yellow-pigmented, moderately halophilic, facultatively alkaliphilic, non-motile, non-spore-forming, catalase- and oxidase-positive, obligately aerobic Gram-positive coccus, strain YIM-C678T was isola...
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Article
Systems-level metabolic flux profiling identifies fatty acid synthesis as a target for antiviral therapy
Munger et al. show that infection with human cytomegalovirus upregulates fatty acid biosynthesis and that pharmacological inhibition of this pathway inhibits replication of both this virus and influenza A. This a...
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Article
Fluoride and Children’s Intelligence: A Meta-analysis
This paper presents a systematic review of the literature concerning fluoride that was carried out to investigate whether fluoride exposure increases the risk of low intelligence quotient (IQ) in China over th...
