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  1. No Access

    Article

    Influences of genetically perturbing synthesis of the typical yellow pigment on conidiation, cell wall integrity, stress tolerance, and cellulase production in Trichoderma reesei

    The prominent protein producing workhorse Trichoderma reesei secretes a typical yellow pigment that is synthesized by a gene cluster including two polyketide synthase encoding genes sor1 and sor2. Two transcripti...

    Weixin Zhang, Ning An, Junqi Guo, Zhixing Wang, **angfeng Meng… in Journal of Microbiology (2021)

  2. No Access

    Protocol

    The Copper-Controlled RNA Interference System in Trichoderma reesei

    Trichoderma reesei is capable of secreting large amounts of lignocellulose-degrading enzymes. Although the genome sequence of T. reesei has been available, the molecular mechanisms of the hyper-production of cell...

    Lei Wang, Weixin Zhang, **angfeng Meng, Weifeng Liu in Trichoderma reesei (2021)

  3. No Access

    Article

    A novel transcriptional regulator RXE1 modulates the essential transactivator XYR1 and cellulase gene expression in Trichoderma reesei

    XYR1 is the key transcription activator for cellulase gene expression in the model filamentous fungus Trichoderma reesei, which is widely applied in the industry due to its excellent capability of secreting a lar...

    Lei Wang, **nxing Lv, Yanli Cao, Fanglin Zheng… in Applied Microbiology and Biotechnology (2019)

  4. No Access

    Article

    Biochemical characterization of a GH70 protein from Lactobacillus kunkeei DSM 12361 with two catalytic domains involving branching sucrase activity

    The fructophilic bacterium Lactobacillus kunkeei has promising applications as probiotics promoting the health of both honey bees and humans. Here, we report the synthesis of a highly branched dextran by L. kunke...

    **angfeng Meng, Joana Gangoiti, **aofei Wang… in Applied Microbiology and Biotechnology (2018)

  5. No Access

    Article

    Biochemical and genetic characterization of fungal proline hydroxylase in echinocandin biosynthesis

    An intriguing structural feature of echinocandins is the incorporation of hydroxylated amino acids. Elucidation of the machinery and the mechanism responsible for this modification is critical to generate new ...

    Feng Zhang, Hui Liu, Tao Zhang, Tjaard Pijning… in Applied Microbiology and Biotechnology (2018)

  6. Article

    Open Access

    Glucansucrase Gtf180-ΔN of Lactobacillus reuteri 180: enzyme and reaction engineering for improved glycosylation of non-carbohydrate molecules

    Glucansucrases have a broad acceptor substrate specificity and receive increased attention as biocatalysts for the glycosylation of small non-carbohydrate molecules using sucrose as donor substrate. However, t...

    Tim Devlamynck, Evelien M. te Poele… in Applied Microbiology and Biotechnology (2016)

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  7. Article

    Open Access

    Structure–function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes

    Lactic acid bacteria (LAB) are known to produce large amounts of α-glucan exopolysaccharides. Family GH70 glucansucrase (GS) enzymes catalyze the synthesis of these α-glucans from sucrose. The elucidation of t...

    **angfeng Meng, Joana Gangoiti, Yuxiang Bai… in Cellular and Molecular Life Sciences (2016)

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  8. No Access

    Article

    Truncation of domain V of the multidomain glucansucrase GTF180 of Lactobacillus reuteri 180 heavily impairs its polysaccharide-synthesizing ability

    Glucansucrases are exclusively found in lactic acid bacteria and synthesize a variety of α-glucans from sucrose. They are large multidomain enzymes belonging to the CAZy family 70 of glycoside hydrolase enzyme...

    **angfeng Meng, Justyna M. Dobruchowska… in Applied Microbiology and Biotechnology (2015)

  9. No Access

    Article

    Evaluation of swollenin from Trichoderma pseudokoningii as a potential synergistic factor in the enzymatic hydrolysis of cellulose with low cellulase loadings

    Swollenin is a novel plant expansin-like protein that has been proposed to have a cellulose disruption activity. In this study, the recombinant swollenin (SWO2) from Trichoderma pseudokoningii S38 was successfull...

    Qingxin Zhou, **nxing Lv, ** Zhang… in World Journal of Microbiology and Biotechn… (2011)