6,486 Result(s)
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Article
The distribution of calmodulin and Ca2+-activated calmodulin in cell cycle of mouse erythroleukemia cells
Cell proliferation is accompanied with changing levels of intracellular caimodulin (CAM) and its activation. Prior data from synchronized cell population could not actually stand for various CaM levels in diff...
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Article
The effects of DNA intercalators on chromatin of chicken red blood cells --- differential extraction on nonhistone proteins
Taking advantage of the effects on DNA secondary structure of two DNA-intercalators, ethidium bromide and chloroquine, we used each of them to treat nuclei from both mature erythrocytes and reticulocytes of ch...
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Construction and packaging of pseudotype retrovirus containing human N-ras cDNA antisense sequence and its biological effects on human hepatoma cells
N-ras is one of the transforming genes in human hepatic cancer cells. It has been found that N-ras was overexpressed at the mRNA and probein level in hepatoma cells. In order to explore the biological roles of N-
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Comparing the frequencies of restriction fragment length polymorphisms for dystrophin gene in Chinese with those from Japanese and Caucasian populations
The restriction fragment length polymorphisms distribution and frequency of dystrophin gene in Chinese were studied by using 14 subclones of the entire 14kb cDNA for the dystrophin as hybridization probes. All...
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Effect of captopril or verapamil on intracellular sodium in cultured vascular smooth muscle cells
The effects of captopril (Cap) and verapamil (Ver) alone and in combination on intracellular Na+ concentration ([Na+]i) in cultured aortic smooth muscle cells(ASMC) of rabbits was evaluated by a direct measuremen...
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Article
Bcl-2 acts upstream of the PARP protease and prevents its activation
Apoptosis has recently been extensively studied and multiple factors have been implicated in its regulation. It remains unclear how these factors are ordered in the cell death pathway. Here we investigate the ...
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Differential expression of a cDNA clone in human liver versus hepatic cancer highly homologous to aryl-dialkylphosphatase
We applied the technique of mRNA differential display to normal liver tissue and hepatoma cell line Hep3B. One of the isolated cDNA clones was expressed in human normal liver tissue but not in the human hepato...
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Enhanced radioimmunotherapeutic efficacy of a monoclonal antibody cocktail against SMMC-7721 human hepatocellular carcinoma
The improved tumoricidal effect of the radioantibody mixture (“cocktail”) has been reported recently for the treatment of colon tumor. In the present study, we demonstrated the enhanced radioimmunotherapeutic ...
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Article
Comparative investigation on spindle behavior and MPF activity changes during oocyte maturation between gynogenetic and amphimictic crucian carp
The spindle behavior and MPF activity changes in the progression of oocyte maturation were investigated and compared with cytological observation and kinase assay between gynogenetic silver crucian carp and am...
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Article
Immunoglobulin-binding proteins in ticks: new target for vaccine development against a blood-feeding parasite
Humans have a long history of trying to control ticks. At first, attempts focused on modifying the habitat, whereas later efforts relied heavily on the use of chemicals. Current research is directed at findin...
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Human μ-opioid receptor overexpressed in Sf9 insect cells functionally coupled to endogenous Gi/o proteins
Human m-opioid receptor (H μ OR) with a tag of six consecutive histidines at its carboxyl terminus had been expressed in recombinant baculovirus infected Sf9 insect cells. The maximal binding capacity for the [3H...
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Article
Cyanide Self-Addition, Controlled Adsorption, and Other Processes at Layered Double Hydroxides
Layered double hydroxides (LDH) are anion-exchangingmaterials of the type M(III)-M(II)x(OH)(2x+2)Y thatoccur abundantly in nature, and can concentrate, protect, andactivate simple organic anionic species of possi...
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Article
Paracrine regulation of apoptosis by steroid hormones in the male and female reproductive system
In males, androgens are essential in maintaining the integrity of the prostate. Androgen-ablation induces apoptosis of the prostatic epithelium. In females, ovariectomy induces apoptosis in uterine epithelium ...
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Article
Costimulation of resting B lymphocytes alters the IL-4-activated IRS2 signaling pathway in a STAT6 independent manner: implications for cell survival and proliferation
IL-4 is an important B cell survival and growth factor. IL-4 induced the tyrosine phosphorylation of IRS2 in resting B lymphocytes and in LPS- or CD40L-activated blasts. Phosphorylated IRS2 coprecipitated with...
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Construction and immunogenicity prediction of Plasmodiumfalciparum CTL epitope minigene vaccine
The minigenes encoding Plasmodiumfalciparum CTL epitopes restricted to human MHC class I molecular HLA-A2 and HLA-B51, which were both at high frequency among Chinese population, were constructed as mono-epitope ...
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Maternal zinc deficiency impairs brain nestin expression in prenatal and postnatal mice
Effects of maternal dietary zinc deficiency on prenatal and postnatal brain development were investigated in ICR strain mice. From d 1 of pregnancy (E0) until postnatal d 20 (P20), maternal mice were fed exper...
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Article
Effect of Inhibitors on the Montmorillonite Clay-Catalyzed Formation of RNA: Studies on the Reaction Pathway
The Langmuir adsorption isotherms of the phosphoroimidazolides of adenosine (ImpA) and uridine (ImpU), dA5'ppdA and N6, N6-dimethyladenine binding on montmorillonite are consistent with their forming a monolayer ...
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Article
Open AccessA genomic timescale for the origin of eukaryotes
Genomic sequence analyses have shown that horizontal gene transfer occurred during the origin of eukaryotes as a consequence of symbiosis. However, details of the timing and number of symbiotic events are uncl...
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The inhibition of lung cancer cell growth by intracellular immunization with LC-1 ScFv
A monoclonal antibody, LC-1, recognizing lung cancer associated common antigens was obtained in authors laboratory. Its single chain Fv fragment (ScFv) named LC-1 ScFv was constructed based on recombinant phag...
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Open AccessAnalysis of a human brain transcriptome map
Genome wide transcriptome maps can provide tools to identify candidate genes that are over-expressed or silenced in certain disease tissue and increase our understanding of the structure and organization of th...
