1,637 Result(s)
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**-Liang Liu
Biomedicine
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Article
Geographical variability of alpha-1-antitrypsin alleles in China: A study on six Chinese populations
Alpha-1-antitrypsin phenotypes of six Chinese Han populations (20°–45°N. latitude) were determined. The frequency of allele M2 increases from North to South China. The north-south change of M2 appears to be ma...
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Article
Ultrastructural sequence of myelin breakdown during Wallerian degeneration in the rat optic nerve
Adult albino rats were subjected to unilateral surgical removal of the eyeball. After survival times of 7–140 days, the numerical response of the neuroglial cells, and the progressive disintegration of the mye...
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Article
Alpha-1-antitrypsin types in five Chinese national minorities
The frequencies of alpha-1-antitrypsin alleles were determined for five Chinese national minorities: Uigur, Korean, Mongolian, Chuang, and Li. PI*S and PI*Z alleles are not found in the five populations studied. ...
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Article
Adenosine Triphosphate Liposomes: Encapsulation and Distribution Studies
Four methods for encapsulating adenosine triphosphate (ATP) in liposomes were evaluated. Optimum entrapment required emulsifying ATP with the lipids used to form the liposome membrane in a high-speed homogeniz...
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The accuracy of prostate cancer staging in a population-based tumor registry and its impact on the Black-White stage difference (Connecticut, United States)
Stage at diagnosis of prostate cancer is a major determinant of survival. Among Blacks, prostate cancer is diagnosed at a later stage of disease than among Whites. This study examined the accuracy of routine c...
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Paranodal structural abnormalities in rat CNS myelin develo**in vivo in the presence of implanted O1 hybridoma cells
O1 hybridoma cells, which produce a monocolonal IgM antigalactocerebroside, were implanted into the spinal cords of immature and mature rats and the cords examined 5–24 days later. Study of the younger group, ...
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Cloning, Expression and Sequence Analysis of the Classical Swine Fever Virus Nucleocapsid Protein
The DNA complementary to the 5′-terminal 1929 nucleotides of classical swine fever virus (CSFV; alias hog cholera virus, HCV) LPC vaccine strain RNA was cloned and sequenced. The sequence encompasses a 5′-nonc...
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Article
Inversion and Transposition of Tc1 Transposon of C. elegans in Mammalian Cells
Tc1/mariner transposons are widespread in the eukaryotes. In vitro transposition test indicated that the transposase is the only protein that is needed in transpositions. It was shown later that ...
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Molecular Cloning and Nucleotide Sequence of 3′-Terminal Region of Classical Swine Fever Virus LPC Vaccine Strain
A cDNA of the 3′-terminus of classical swine fever virus (LPC vaccine strain) was cloned and sequenced. The 3431 nucleotides and deduced amino acid sequences were compared with those of other pestiviruses, and...
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Article
Immunotherapy of tumors with xenogeneic endothelial cells as a vaccine
The breaking of immune tolerance against autologous angiogenic endothelial cells should be a useful approach for cancer therapy. Here we show that immunotherapy of tumors using fixed xenogeneic whole endotheli...
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Article
Wildtype Kras2 can inhibit lung carcinogenesis in mice
Although the ras genes have long been established as proto-oncogenes, the dominant role of activated ras in cell transformation has been questioned. Previous studies have shown frequent loss of the wildtype Kras2
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Cloning and Sequencing of Full-length cDNA of Classical Swine Fever Virus LPC Strain
The cDNAs of classical swine fever virus (LPC vaccine strain) were cloned by transcriptase-polymerase chain reaction, and their nucleotide sequences were determined. In this work, we obtained the sequence info...
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Article
Prospects of chimeric RNA-DNA oligonucleotides in gene therapy
A strategy called targeted gene repair was developed to facilitate the process of gene therapy using a chimeric RNA-DNA oligonucleotide. Experiments demonstrated the feasibility of using the chimeric oligonucl...
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Article
Insulator: from chromatin domain boundary to gene regulation
An insulator or boundary element is a novel class of regulatory sequence and has properties consistent with a role in limiting enhancer activity; it helps to define the boundary between differentially regulat...
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Article
Induction of chemosensitivity in nasopharyngeal carcinoma cells using a human papillomavirus regulatory sequence and the thymidine kinase gene
Nasopharyngeal carcinoma (NPC) is a human cancer of epithelial cell origin. Infection by Epstein-Barr virus has been shown to be closely associated with this tumor. Recent studies have indicated that another c...
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Article
Analysis of adeno-associated virus-mediated ex vivo transferred human β-globin gene in bone marrow engrafted mice
Adeno-associated virus (AAV)-2 was developed as a useful vector for human gene therapy. In this report, we analyzed the integration and expression of AAV-mediated ex vivo transferred human β-globin gene in bon...
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Oligonucleotide-mediated gene repair at DNA level: the potential applications for gene therapy
Mutations in gene sequence can cause many genetic disorders, and researchers have attempted to develop treatments or cures at the DNA level for these diseases. Several strategies including triple-helix-formin...
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Article
Buspirone-induced antinociception is mediated by l-type calcium channels and calcium/caffeine-sensitive pools in mice
Previous studies have shown that buspirone, a partial 5-HT1A receptor agonist, produces antinociceptive effects in rats and mice; Ca2+ plays a critical role as a second messenger in mediating nociceptive transmis...
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The effect of metal ions on the binding of ethanol to human alcohol dehydrogenase β2β2
Molecular docking simulations were performed in this study to investigate the importance of both structural and catalytic zinc ions in the human alcohol dehydrogenase β2β2 on substrate binding. The structural zin...
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Gender difference in ranolazine pharmacokinetics in rats
Pharmacokinetics of ranolazine(RAN) in both female and male rats was studied. RAN concentrations in plasma were determined after oral administration of 12.5,25 and 50 mg/kg RAN. Concentrations in tissues, reco...
