Search
Search Results
-
Sustainability in Biobanking
Biobanks are storage places for biospecimens that can be used for current and future scientific research. Biospecimens are exceptional sources of...
-
Procurement and Storage of Surgical Biospecimens
A biobank is an important nexus between clinical and research aspects of pathology. The collection and storage of high quality surgical samples is...
-
Minority Participation in Biobanks: An Essential Key to Progress
Biobanks are critical resources for biomedical research and will be a driving force behind personalized medicine. Although biobanking efforts are...
-
Antigen-Specific Detection of Autoantibodies Against Myeloperoxidase (MPO) and Proteinase 3 (PR3)
ANCA testing was introduced in many laboratories throughout the world when it was recognized that a significant subset of patients with small vessel...
-
Gene Transfer of Calcium-Binding Proteins into Adult Cardiac Myocytes
Heart failure is the leading cause of combined morbidity and mortality in the USA with 50% of cases being diastolic heart failure. Diastolic heart...
-
Three-Dimensional Reconstruction Imaging Method to Study the Function of EFHD2 in Invadopodia Formation
Calcium and calcium-binding proteins play crucial roles in the regulation of actin dynamics, which contributes to cancer cell migration and invasion....
-
Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults
In recent years there have been numerous attempts to establish reliable and consistent noninvasive biomarkers of gut inflammation. Here we describe...
-
S100A7 in Psoriasis: Immunodetection and Activation by CRISPR technology
Psoriasis, an inflammatory autoimmune skin disease, is the result of a chronic interaction between hyperproliferative keratinocytes and infiltrating...
-
Antisense Oligonucleotides for Splice Modulation: Assessing Splice Switching Efficacy
Today, there are emerging numbers of oligonucleotide therapies being approved by the governmental authorities. These types of therapies present a...
-
Attachment of Peptides to Oligonucleotides on Solid Support Using Copper(I)-Catalyzed Huisgen 1,3-Dipolar Cycloaddition
In vivo bioavailability and delivery of nucleic acids to the site of action is a severe limitation in oligonucleotide (ON) therapeutics. Equip**...
-
Click-Shielded and Targeted Lipopolyplexes
Lipopolyplexes present well-established nucleic acid carriers assembled from sequence-defined cationic lipo-oligomers and DNA or RNA. They can be...
-
Biobanking of Cerebrospinal Fluid
Cerebrospinal fluid (CSF) is a physiologically essential fluid produced by the brain that is involved in protecting the brain and in the exchange of...
-
Laboratory Safety: Chemical and Physical Hazards
Chemical and physical hazards are part of the daily routine when working in biorepositories. This chapter provides a brief overview of some common...
-
An Introduction to Performing Immunofluorescence Staining
Immunofluorescence (IF) is an important immunochemical technique that allows for detection and localization of a wide variety of antigens in...
-
Domestic and International Ship** of Biospecimens
The packaging and shipment of biospecimens is a multistep process for which a distinct set of regulations needs to be followed, depending on whether...
-
An Introduction to the Performance of Immunohistochemistry
Immunohistochemistry (IHC) is a powerful technique that exploits the specific binding between an antibody and antigen to detect and localize specific...
-
Collection and Preservation of Terrestrial Arthropods
Arthropods comprise an amazingly diverse group of life forms that are extensively studied in almost every field of the biological sciences. Given...
-
Analysis of C3 Nephritic Factors by ELISA
Autoantibodies to the C3 convertase of the alternative pathway of complement, called C3 nephritic factors (C3NeF), cause persistently low C3 in the...
-
Determination of Rheumatoid Factors by ELISA
IgM and IgA autoantibodies binding to IgG are called rheumatoid factors (RFs) and occur with high frequency in rheumatoid arthritis (RA) and with...
-
Determination of Autoantibodies to Salivary Gland Antigens
Primary Sjögren’s syndrome (pSS) is a systemic autoimmune disease marked by dry mouth/dry eye symptoms as a result of the destruction of the salivary...