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Luminescence-Based Techniques for KRAS Thermal Stability Monitoring
Various biochemical methods have been introduced to detect and characterize KRAS activity and interactions, from which the vast majority is based on...
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Progress in Targeting KRAS Directly
RAS research has entered the world of translational and clinical science. Progress has been based on our appreciation of the role of RAS mutations in...
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Metabolomic Investigations into Hypoxia-Mediated Metabolic Reprogramming of Pancreatic Cancer Cells
Hypoxia is a crucial microenvironmental factor that defines tumor cell growth and aggressiveness. Cancer cells adapt to hypoxia by altering their...
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Staining Hypoxic Areas of Frozen and FFPE Tissue Sections with Hypoxyprobe™
Hypoxia occurs due to inadequate levels of oxygen in tissue and has been implicated in numerous diseases such as cancer, diabetes, cardiovascular,...
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Measurement of Metabolic Alteration in Immune Cells Under Hypoxia
The hypoxic microenvironment in solid tumors affects the metabolism of tumor cells and infiltrating immune cells, which aids in robust tumor growth...
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Genetically Encoded Reporters to Monitor Hypoxia
Hypoxia resulting from an imbalance of oxygen availability and consumption defines a metabolic cellular state with a profound impact on developmental...
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Oxomer- and Reporter Gene-Based Analysis of FIH Activity in Cells
Cellular and tissue adaptations to oxygen deprivation (hypoxia) are necessary for both normal physiology and disease. Responses to hypoxia are...
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Application of a Specific and Sensitive NQO1 Turn-On Near-Infrared Fluorescence Probe for Live Cancer Cell and Xenografted Tumor Imaging in Nude Mice
Sensitive activity stains for enzymes selectively expressed in human cancers offer valuable tools for imaging with wide applications in experimental,...
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Evaluation of Oxygen Consumption Rates In Situ
An analysis of the mitochondrial respiration function represented by the oxygen consumption rate is necessary to assess mitochondrial bioenergetics...
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Oxygen Assessment in Tumors In Vivo Using Phosphorescence Lifetime Imaging Microscopy
The oxygen level in a tumor is a crucial factor for its development and response to therapies. Phosphorescence lifetime imaging (PLIM) with the use...
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Measuring Pericellular Oxygen Tension for In Vitro Cell Culture
Cell culture, the process of growing cells in conditions that mimic those in the body, is a key technique in biomedical research. Oxygen is not...
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Establishment of Mouse Orthotopic Urinary Bladder Tumor Model and Its Analysis by Light and Electron Microscopy
Mouse tumor models are an important tool in cancer research, and the orthotopic cancer cell transplantation model is the most widely used among them....
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In Vivo Wound Healing Model for Characterization of Gene Electrotransfer Effects in Mouse Skin
Wound healing is a complex biological response to injury characterized by a sequence of interdependent and overlap** physiological actions. To...
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In Vivo Bioluminescence and Fluorescence Imaging: Optical Tool for Cancer Research
In vivo whole-body imaging, using optical tools based on bioluminescence and fluorescence detection, offers tremendous opportunities to specifically...
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Kinetic Modeling of Hepatic Metabolism and Simulation of Treatment Effects
Mathematical modeling is a promising strategy to fill the experimentally unapproachable knowledge gaps about the relative contribution of various...
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Flow Cytometry Assessment of Lymphocyte Populations Infiltrating Liver Tumors
Tissue-resident and recruited immune cells are essential mediators of natural and therapy-induced immunosurveillance of liver neoplasia. This idea...
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Diethylnitrosamine Induction of Hepatocarcinogenesis in Mice
Diethylnitrosamine (DEN) is a chemical hepatocarcinogenic agent that triggers a large array of oncogenic mutations after a single injection....
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Diethylnitrosamine-Induced Liver Tumorigenesis in Mice Under High-Hat High-Sucrose Diet: Stepwise High-Resolution Ultrasound Imaging and Histopathological Correlations
The hepatotoxic N-nitroso compound diethylnitrosamine (DEN) administered intraperitoneally (i.p.) induces liver neoplasms in rodents that...
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In Vivo Modeling of Leukemia by Murine Stem Cell Transplantation
Murine stem cell transplantation is a well-established method for the in vivo study of leukemic pathophysiology. Adoptive transfer of murine leukemic...
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Cancer Stem Cells: Current Challenges and Future Perspectives
Despite major advances in health care including improved diagnostic tools, robust chemotherapeutic regimens, advent of precision, adjuvant and...