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Droplet Microfluidics with Capillary Electrophoresis for Glycan Biomarker Analysis
Several glycoproteins are validated biomarkers of various diseases such as cancer, cardiovascular diseases, chronic alcohol abuse, or congenital...
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Microchip for Immunomagnetic Sorting of Circulating Tumor Cells (CTCs)
Circulating tumor cells (CTCs) isolated directly from whole blood opens new perspectives for cancer monitoring and the development of personalized...
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Mutation Screening of Dictyostelium Restriction Enzyme-Mediated Integration (REMI) Libraries
Identifying the mechanisms of action of existing and novel drugs is essential for the development of new compounds for therapeutic and commercial...
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Quantitative Analysis of the Inactivation Process of Internalized Bacteria in Dictyostelium Cells
The understanding of the inactivation process of ingested bacteria by phagocytes is a key focus in the field of host-pathogen interactions....
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Analysis of cGMP Signaling in Dictyostelium
Biochemical assays are described to analyze signal transduction by the second messenger cGMP in Dictyostelium. The methods include enzyme assays to...
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Single Cell Transcriptome Analysis During Development in Dictyostelium
Dictyostelium represents a stripped-down model for understanding how cells make decisions during development. The complete life cycle takes around a...
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Methods to Assess Autophagic Activity in Dictyostelium
Autophagy is an intracellular clearance and recycling pathway that delivers different types of cargos to lysosomes for degradation. In recent years,...
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Synthesis of Azo Analogs for Investigating Phosphatidic Acid-Mediated Signaling
Phosphatidic acid (PA) is a key signaling lipid that plays a crucial role in regulating various cellular processes. Studies have shown that...
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Analysis of Lipid GPCR Molecular Interactions by Proximity Labeling
G-protein-coupled receptors (GPCRs) are hepta-helical transmembrane proteins that mediate various intracellular signaling events in response to their...
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Zebrafish as a Model for Lipidomics and Similar Investigations
Zebrafish (Danio rerio) has emerged as a pivotal model organism in vertebrate development research over several decades. Beyond its contributions to...
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Optimized Lipidomics Extraction of Sphingosine and Sphinganine from Optic Nerve for Signaling Studies
Interconvertible sphingolipid metabolites represent germane constituents of eukaryotic membranes and are vital in the regulation of cellular...
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Market Needs and Methodologies Associated with Patient Lipidomic Diagnoses and Analyses
This chapter conducts an in-depth exploration of the impact of musculoskeletal (MSK) disorders and injuries, with a specific emphasis on their...
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α-Linolenic Acid Vesicles-Mediated Tau Internalization in Microglia
In Alzheimer’s disease, the synaptic loss is prominent due to the accumulation of Amyloid βeta (Aβ) protein in synapses, which affect...
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Oxidized Low-Density Lipoprotein: Preparation, Validation, and Use in Cell Models
Lipoproteins in plasma are constituted by the least dense chylomicron, very-low-density lipoprotein (VLDL), low-density lipoprotein (LDL), and...
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Analysis of Lipoprotein Signaling in Iris Melanocytes
Lipids are compounds involved in many biologic functions including cell structure, metabolism, energy storage and are involved in signaling. A...
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In Situ Imaging of Proteins Using DNA-PAINT Super-Resolution Microscopy
The spatial resolution of conventional light microscopy is restricted by the diffraction limit to hundreds of nanometers. Super-resolution microscopy...
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Imaging Cell Signaling in Tissues Using the IBEX Method
The study of cell signaling within tissues can be enhanced using highly multiplexed immunohistochemistry to localize the presence and spatial...
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Deep Learning-Based Cell Tracking in Deforming Organs and Moving Animals
Cell tracking is an essential step in extracting cellular signals from moving cells, which is vital for understanding the mechanisms underlying...
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Single-Cell Measurements Using Acoustic Force Spectroscopy (AFS)
Single-molecule force spectroscopy is a powerful tool to investigate the forces and motions related to interactions of biological molecules. Acoustic...
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Unfolding and Refolding Proteins Using Single-Molecule AFM
Single-molecule atomic force microscopy (AFM) allows capturing the conformational dynamics of an individual molecule under force. In this chapter, we...