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Protocorm-Like Body Induction and Development Using In Vitro Culture Techniques and Biotechnological Methods
The ability to form protocorm-like bodies (PLBs) is a characteristic regeneration feature of orchid explants. Micropropagation methods using defined...
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Genome Editing to Produce Knockout Mutations of Seed Dormancy Genes in Wheat
Knockout mutants provide definitive information about the functions of genes related to agronomic traits, including seed dormancy. However, it takes...
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Regulation of Seed Dormancy Genes in Triticeae Species
Wild progenitors of Triticeae crops generally have long dormancy periods. Domesticated crops inherited these longer dormancy alleles from their wild...
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Chromatin Immunoprecipitation Using Imbibed Seeds of Arabidopsis thaliana
Chromatin immunoprecipitation (ChIP) is used to analyze the targeting of a protein to a specific region of chromatin in vivo. Here, we present an...
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Microtome-Based Sectioning Method for Generating Thin Cell Layer Cultures in Phalaenopsis
The production of virus-free plants is crucial in the supply chain management in the international trade of orchids. Excising the shoot tip from...
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Transplant and Care for In Vitro Orchid Seedlings
Transferring young orchid seedlings from in vitro culture to the external environment is critical for orchid micropropagation. This chapter...
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Dendrobium candidum Wall. ex Lindl.: Production of Protocorm-Like Body (PLB) Biomass and Bioactive Compounds
Dendrobium candidum Wall ex Lindl. is a popular medicinal orchid widely used in traditional Chinese medicine. This species has become rare in nature,...
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In Vitro Asymbiotic and Symbiotic Germination Techniques for Terrestrial Orchid Propagation
Orchids produce numerous tiny seeds that require external nutrients to grow and develop into mature plants. In their natural habitat, this...
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Transcriptomics Analysis in the Study of Mycorrhizal Symbiosis in Orchids
In past decades, high-throughput transcriptome sequencing has been widely applied to explore the transcript control of the gene expression during the...
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CRISPR-Cas9 Technology in Plant Genomics Research: A Review and Tutorial for Implementation with Considerations for Orchid Genetic Studies
The study and identification of gene mutations resulting in alterations to plant phenotype form the basis of plant genetic screens. Assigning...
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Isolation of Protoplasts from Tissues of Mexican Prickly Poppy (Argemone mexicana L.): An Alkaloid-Producing Medicinal Plant
Protoplasts are plant cells from which the pectocellulosic cell wall has been removed, thus kee** the plasma membrane intact. For plant secondary...
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Micropropagation of the Ornamental Aquatic Plant, Aponogeton ulvaceus, from Immature Tuber Explants
The aquatic monocot, Aponogeton ulvaceus Baker, is endemic to Madagascar and is a commercially valuable ornamental aquarium plant. Members of the...
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Plant Micropropagation and Temporary Immersion Systems
Temporary immersion systems (TIS) have been widely recognized as a promising technology for micropropagation of various plant species. The TIS...
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Use of Statistics in Plant Cell Tissue Culture
Statistics and experimental design are important tools for plant cell and tissue culture researchers and should be used when planning and conducting...
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Purification of Recombinant N-terminal Histidine-Tagged Arabidopsis thaliana Phosphoglycolate Phosphatase 1, Glycolate Oxidase 1 and 2, and Hydroxypyruvate Reductase 1
To measure the kinetic properties of photorespiratory enzymes, it is necessary to work with purified proteins. Protocols to purify photorespiratory...
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Mitochondrial Dihydrolipoamide Dehydrogenase (mtLPD1): Expression, Purification, Activity, and Redox Regulation
Mitochondrial dihydrolipoamide dehydrogenase (mtLPD1) is a central enzyme in primary carbon metabolism, since its function is required to drive four...
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Combining Gas Exchange and Rapid Quenching of Leaf Tissue for Mass Spectrometry Analysis Directly in Gas Exchange Cuvette
Isotopically nonstationary metabolic flux analysis (INST-MFA) is a powerful technique for studying plant central metabolism, which involves...
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Measuring Phosphoglycolate Phosphatase Activity Using NMR Detection of Glycolate
Besides the historical and traditional use of nuclear magnetic resonance (NMR) spectroscopy as a structure elucidation tool for proteins and...
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Using Gas Exchange to Study CO2 Release During Photosynthesis with Steady- and Nonsteady-State Approaches
Measures of respiration in the light and Ci* are crucial to the modeling of photorespiration and photosynthesis. This chapter provides background on...
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Reaction-Kinetic Modeling of Photorespiration Using Modelbase
Plant science has become more and more complex. With the introduction of new experimental techniques and technologies, it is now possible to explore...