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Correction to: Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes Using miRcombo
This chapter was previously published as non-Open Access. Under the request of the authors the chapter is now converted to Open Access and is...
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Cardiac Gene Delivery in Large Animal Models: Selective Retrograde Venous Injection
Delivery of viral vectors to the heart represents a challenging endeavor. Besides vector design, the route of substrate administration is...
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Endobronchial Gene Delivery for Pulmonary Hypertension in a Large Animal Model
Pulmonary hypertension (PH)Pulmonary hypertension (PH) is a devastating disease with high morbidity and mortality. Despite significant progress in...
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CRISPR/Cas9 Gene Editing of RYR2 in Human iPSC-Derived Cardiomyocytes to Probe Ca2+ Signaling Aberrancies of CPVT Arrhythmogenesis
Human-induced pluripotent stem cells (hiPSCs) provide a powerful platform to study biophysical and molecular mechanisms underlying the...
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Generation of Atrial-Specific Construct Using Sarcolipin Promoter-Associated CRM4 Enhancer
Cardiac gene therapy has been hampered by off-target expression of gene of interest irrespective of variety of delivery methods. To overcome this...
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Updates on Cardiac Gene Therapy Research and Methods: Overview of Cardiac Gene Therapy
Gene therapy has made a significant progress in clinical translation over the past few years with several gene therapy products currently approved or...
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Left Ventricular Pressure Volume Assessment Using Carotid Artery Access in the Rat
Left ventricular (LV) catheterization with either a pressure catheter or pressure–volume catheter provides a means to measure cardiac function, an...
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ELISpot Assay for Gene Therapy in Large Animal Studies
Formation of neutralizing antibodies and cellular immune response with repeat adeno-associated virus (AAV) gene therapy dosing are critical concerns...
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Next-Generation Sequencing
Next-generation sequencing (NGS) technologies refer to the high-throughput sequencing techniques. “Next-generation” sequencing technologies lead to...
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Conventional and Basic Genomic Techniques
In this chapter, certain very basic and primary technologies pertaining to genomics have been recapitulated. The topics covered include basic...
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DNA Microarray
Microarray is a hybridization-based technique where a greater number of genes can be studied at a time. Each microchamber will be allotted to...
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RNA Sequencing (RNA-seq)
RNA sequencing (or popularly known as RNA-seq) or whole transcriptome shortgun sequencing (WTSS) is a useful next-generation sequencing (NGS)...
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Introduction to Bioinformatics
Bioinformatics is an interdisciplinary field using dry lab, that is, methods and software tools for understanding biological data. Bioinformatics...
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Exome Sequencing
Exome sequencing, also known as whole exome sequencing (WES or WXS), is a technique for sequencing all the expressed genes in a genome (known as the...
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Big Data Analysis
Big data is a term applicable to data that are too large or complex for traditional data-processing...
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Expressing Cloned Genes for Protein Production, Purification, and Analysis-Biopharming
Protein expressed in vitro through expression vector is very important for therapeutic agent, gene therapy, and transgenesis. There are certain...
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Rapid Amplification of cDNA Ends (RACE)
Rapid amplification of cDNA ends (RACE) or “one-sided” polymerase chain reaction (PCR) or “anchored” PCR is a polymerase chain reaction-based...
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Cardiac Targeted Adeno-Associated Virus Injection in Rats
Gene therapy is a promising approach in the treatment of cardiovascular diseases. The vectors available for cardiovascular gene therapy have...
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Assessing the Effect of Cardiac Gene Therapy Using Catheter-Based Pressure–Volume Measurement in Large Animals
Gene therapy for heart failure targets various pathways that modulate cardiac function. Its detailed evaluation is crucial for proving the efficacy...
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Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes Using miRcombo
Direct reprogramming of fibroblasts into induced cardiomyocytes (iCMs) through microRNAs (miRNAs) is a new emerging strategy for myocardial...