Search
Search Results
-
Genetic Network Design Automation with LOICA
Genetic design automation (GDA) is the use of computer-aided design (CAD) in designing genetic networks. GDA tools are necessary to create more...
-
Cell-Free Synthesis and Quantitation of Bacteriophages
Cell-free transcription-translation (TXTL) enables achieving an ever-growing number of applications, ranging from the rapid characterization of DNA...
-
The Causes for Genomic Instability and How to Try and Reduce Them Through Rational Design of Synthetic DNA
Genetic engineering has revolutionized our ability to manipulate DNA and engineer organisms for various applications. However, this approach can lead...
-
Multiplex Functional Characterization of Protein Variant Libraries in Mammalian Cells with Single-Copy Genomic Integration and High-Throughput DNA Sequencing
Sequencing-based, massively parallel genetic assays have enabled simultaneous characterization of the genotype–phenotype relationships for libraries...
-
Multiplex Marker-Less Genome Integration in Pichia pastoris Using CRISPR/Cas9
Pichia pastoris is known for its excellent protein expression ability. As an industrial methyl nutritional yeast, it can effectively utilize methanol...
-
Multimodal Control of Bacterial Gene Expression by Red and Blue Light
By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility,...
-
Posttranslational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells
Physical cues such as light, heat, or an electrical field can be utilized for traceless, on-demand activation of the expression of a desired...
-
High-Throughput Spectroscopic Analysis of mRNA Cap** Level
Eukaryotic mRNAs are characterized by terminal 5′ cap structures and 3′ polyadenylation sites, which are essential for posttranscriptional...
-
A Directed Evolution Protocol for Engineering Minimal Transcription Factors, Based on CIS Display
Directed evolution is an efficient strategy for obtaining desired biomolecules. Since the 1990s, the emergence of display techniques has enabled...
-
Imaging S-Adenosyl Methionine Dynamics in Living Cells Using an RNA-Based Fluorescent Sensor
S-Adenosyl methionine (SAM) is a critical metabolite involved in numerous cellular processes, including DNA methylation and gene expression...
-
Detection of MicroRNAs Using Synthetic Toehold Switch in Mammalian Cells
Engineering synthetic gene circuits to control cellular functions has a broad application in the field of synthetic biology. Synthetic RNA-based...
-
Protocols for Building and Producing High Diversity Peptide Phage Display Libraries
Phage display is an important technology to study protein-protein interaction and protein evolution, with applications in basic science and applied...
-
Purification of Single-Stranded RNA Bacteriophages and Host Receptors for Structural Determination Using Cryo-Electron Microscopy
Single-stranded RNA bacteriophages (ssRNA phages) are small viruses with a compact genome (~3–4 kb) that infect gram-negative bacteria via retractile...
-
Genome Editing, Transcriptional Regulation, and Forward Genetic Screening Using CRISPR-Cas12a Systems in Yarrowia lipolytica
Class II Type V endonucleases have increasingly been adapted to develop sophisticated and easily accessible synthetic biology tools for genome...
-
Coiled-Coil Interaction Toolbox for Engineering Mammalian Cells
Protein interactions play a crucial role in a variety of biological processes. Therefore, regulation of these interactions has received considerable...
-
Combining a Base Deaminase Mutator with Phage-Assisted Evolution
Phage-assisted evolution has emerged as a powerful technique for improving a protein’s function by using mutagenesis and selective pressure. However,...
-
A Computational Modeling Approach for the Design of Genetic Control Systems that Respond to Transcriptional Activity
Recent progress in synthetic biology has enabled the design of complex genetic circuits that interface with innate cellular functions, such as gene...
-
Generation of a Naïve Human scFv Phage Display Library and Panning Selection
Phage display antibody libraries have been successfully used as the essential tool to produce monoclonal antibodiesMonoclonal antibodies (mAB)...
-
Droplet Microfluidics with Capillary Electrophoresis for Glycan Biomarker Analysis
Several glycoproteins are validated biomarkers of various diseases such as cancer, cardiovascular diseases, chronic alcohol abuse, or congenital...
-
Microchip for Immunomagnetic Sorting of Circulating Tumor Cells (CTCs)
Circulating tumor cells (CTCs) isolated directly from whole blood opens new perspectives for cancer monitoring and the development of personalized...