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Generating Ice-Binding Protein–Polymer Bioconjugates
Protein–polymer conjugates combine the stability of polymers with the diversity, specificity, and functionality of proteins. The resulting hybrid...
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Large-Scale Purification of Natural Ice-Binding Proteins by the Falling Water Ice Purification Method
We describe here a generic technique for purification of ice-binding proteins (IBPs) using a commercial ice machine. The method, which is called...
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Multiscale Molecular Dynamics Simulations of Ice-Binding Proteins
Ice-binding proteins (IBPs) are a diverse class of proteins that are essential for the survival of organisms in cold conditions. IBPs are diverse in...
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Investigating the Interaction Between Ice-Binding Proteins and Ice Surfaces Using Microfluidic Devices and Cold Stages
Ice-binding proteins (IBPs) protect organisms living in sub-freezing conditions by inhibiting ice growth in fish and insects, limiting ice...
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A Liquid Chromatography-Tandem Mass Spectrometry Assay for Quantification of Ethyl Glucuronide and Ethyl Sulfate in Urine
Ethyl glucuronide and ethyl sulfate emerged as the biomarkers of choice for detection of ethanol use as the required sample is urine, enabling easy...
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Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Plasma Busulfan
Busulfan is an alkylating agent and functions as a myeloablative and anti-leukemic chemotherapy drug. It is widely used with cyclophosphamide for...
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Triazole Antifungal Quantification for Therapeutic Drug Monitoring in Serum by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS): Posaconazole, Voriconazole, Itraconazole, and Hydroxyitraconazole
Antifungal therapy with triazole drugs including posaconazole, voriconazole, itraconazole, and its active metabolite hydroxyitraconazole is routinely...
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Liquid Chromatography-Tandem Mass Spectrometry-Based Therapeutic Monitoring of Plasma Atovaquone Concentrations in Pediatric Patients
Atovaquone is an FDA-approved antiparasitic and antifungal therapeutic that is currently used as a prophylactic agent to prevent Pneumocystis carinii...
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Identifying Transmembrane Interactions in Receptor Protein Tyrosine Phosphatase Homodimerization and Heterodimerization
Receptor protein tyrosine phosphatases (RPTPs) are one of the key regulators of receptor tyrosine kinases (RTKs) and therefore play a critical role...
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Preparation of Tau Condensates by Liquid–Liquid Phase Separation to Study Tau Amyloid Aggregation
Protein liquid–liquid phase separation (LLPS) has been associated with protein amyloid aggregation. Amyloid aggregation of tau is a hallmark of...
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Characterization of Posttranslationally Modified PHF-1 Tau Peptides Using Gaussian Accelerated Molecular Dynamics Simulation
The microtubule-associated proteinMicrotubulemicrotubule-associated proteins, Tau, is an intrinsically disordered protein that plays a crucial role...
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Tracking Tau in Neurons: How to Transfect and Track Exogenous Tau in Primary Neurons
Primary murine neurons have proved to be an essential tool for the general investigation of neuronal polarity, polarized Tau distribution, and...
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Assays for the Screening and Characterization of Tau Aggregation Inhibitors
Aggregation of tau protein is a pathological hallmark of Alzheimer’s disease and other neurodegenerative tauopathies. Inhibition of tau aggregation...
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Genetic Network Design Automation with LOICA
Genetic design automation (GDA) is the use of computer-aided design (CAD) in designing genetic networks. GDA tools are necessary to create more...
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Cell-Free Synthesis and Quantitation of Bacteriophages
Cell-free transcription-translation (TXTL) enables achieving an ever-growing number of applications, ranging from the rapid characterization of DNA...
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The Causes for Genomic Instability and How to Try and Reduce Them Through Rational Design of Synthetic DNA
Genetic engineering has revolutionized our ability to manipulate DNA and engineer organisms for various applications. However, this approach can lead...
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Solubilization of Oligomeric Cell-Free Synthesized Proteins Using SMA Copolymers
Although membrane proteins are abundant in nature, their investigation is limited due to bottlenecks in heterologous overexpression and consequently...
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Considerations for Glycoprotein Production
This chapter is intended to provide insights for researchers aiming to choose an appropriate expression system for the production of recombinant...
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Multiplex Functional Characterization of Protein Variant Libraries in Mammalian Cells with Single-Copy Genomic Integration and High-Throughput DNA Sequencing
Sequencing-based, massively parallel genetic assays have enabled simultaneous characterization of the genotype–phenotype relationships for libraries...
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Unnatural Amino Acid Engineering for Intracellular Delivery of Protein Therapeutics
Protein drugs are a critically important therapeutic modality due to the sophisticated binding recognition, catalytic properties, and disease...