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Food Allergens of Plant and Animal Origin: Classification, Characteristics, and Properties
Food allergy is an adverse immune response to specific foods that can be either IgE-mediated or non-IgE mediated. The causes of IgE-mediated food...
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Structural Characterization of Food Allergens by Nuclear Magnetic Resonance Spectroscopy
As allergies, especially those triggered by food, are becoming more and more prevalent, it is of increasing importance to fully understand the...
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A New Microfluidic Device to Facilitate Functional Precision Medicine Assays
Functional precision medicine (FPM) has emerged as a new approach to improve cancer treatment. Despite its potential, FPM assays present important...
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Bulk RNA-seq Assessment of Murine Spleen Using a Portable MinION Sequencing Device
Comprehensive profiling of the transcriptome reflects the dynamic changes in complex regulator interactions between genes and proteins in the...
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16S rRNA Gene Amplicon Analysis of Human Gut Microbiota
The intestinal microbiota is associated with a variety of diseases, and there are a growing number of research reports on the gut microbiota. In...
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Institutional Review Board Considerations for Clinical Trials
To protect subjects who participate in human research, Institutional Review Boards (IRBs) play an important role in reviewing research and...
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Four-Color ImmunoSpot® Assays Requiring Only 1–3 mL of Blood Permit Precise Frequency Measurements of Antigen-Specific B Cells-Secreting Immunoglobulins of All Four Classes and Subclasses
The B lymphocyte response can encompass four immunoglobulin (Ig) classes and four IgG subclasses, each contributing fundamentally different effector...
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Preparation of Infectious Wolbachia from a Mosquito Cell Line
Eventual genetic engineering of Wolbachia will require maximizing recovery of infectious bacteria, maintaining Wolbachia in a viable state for...
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Detection, Quantification, and Elimination of Wolbachia in Bed Bugs
Wolbachia is an obligatory nutritional symbiont of the common bed bug, Cimex lectularius, providing B-vitamins to its host. The biological...
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Engineering Probiotic E. coli Nissle 1917 for Release of Therapeutic Nanobodies
Bioengineered probiotics enable new opportunities to improve cancer treatment strategies due to their tumor-colonizing capabilities. Here, we will...
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Monitoring Cell Cytoskeleton Variations upon Piezoelectric Stimulation: Implications for the Immune System
Piezoelectric stimulation can have a significant impact on different cellular functions with possible applications in several fields, such as...
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Lentiviral Production Platform
Lentiviral-mediated transfection technique is a powerful tool for gene modification in preclinical studies. By using this technique, the desired gene...
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Assessment of Disease Activity, Structural Damage, and Function in Rheumatoid Arthritis
The primary goal in the treatment of rheumatoid arthritis (RA) is to control disease activity, prevent structural damage in joints, and normalize...
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Evaluation of Autoreactive Responses
Loss of tolerance to self-antigens is considered to be one of the initial reasons for the onset of rheumatoid arthritis (RA). Identification of...
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Preparation of Joint Extracts
Since mice are widely used to establish rheumatoid arthritis models, assessment of the pathogenesis of local arthritis is fundamental. Proteins are...
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Biosensors for the Detection of Food Allergens
Biosensors enable fast and specific detection of various molecules, including allergens. Surface plasmon resonance (SPR) aptasensors combine the...
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Production of Neutralizing Antibody
Techniques employing monoclonal antibodies (mAbs) are widely used in the initial development phase of biologics. The usefulness of mAbs in basic RA...
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Reconstruction of Protein/Liposome Complex
Most ion channels and receptors are distributed in cell membranes and are known as membrane proteins. These membrane proteins are folded in the cell...
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Denervation-Induced Sarcopenia Model
Rheumatoid arthritis (RA) is an important risk factor for sarcopenia. Physical inactivity, systemic inflammatory factors, and medication directly or...
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Animal Models of Vasculitis
The diagnosis of vasculitis in rheumatoid arthritis (RV) is associated with considerable mortality; therefore, understanding the basic mechanisms...