26 Result(s)
within
Protocol
Zhuo** **e
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Protocol
Dynamic Proximity Tagging in Living Plant Cells with Pupylation-Based Interaction Tagging
Identification of protein–protein interactions (PPIs) and protein kinase substrates is fundamental for understanding how proteins exert biological functions with their partners and targets. However, it is stil...
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Analysis of Protein Degradation in Ferroptosis
The ubiquitin-proteasome system (UPS) is a highly conserved cellular mechanism that degrades and recycles proteins in eukaryotic cells. It involves the tagging of specific target proteins with ubiquitin, a sma...
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Structural Characterization of Silk Fibers by Small-Angle X-Ray Scattering
Nature is rich in all kinds of unbelievably designed microstructures, which endows the natural materials with various fantastic performances. Unveiling the mystery of the sophisticated configurations and the r...
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Structural Characterization of Silk Fibers by Wide-Angle X-Ray Scattering
As one of the most advanced techniques to gain insight into the structure of the materials, wide-angle X-ray scattering (WAXS) records the scattering information at wide angles which typically larger than 5° (2θ)...
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Selecting Channelrhodopsin Constructs for Optimal Visual Restoration in Differing Light Conditions
Channelrhodopsin (ChR)-based optogenetics is one promising approach to restore vision in photoreceptor degenerative diseases such as retinitis pigmentosa (RP) and age-related macular degeneration (AMD). Curren...
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Characterization of Arsenic-Induced Cancer Stem-Like Cells
Arsenic is a well-known human carcinogen. However, the mechanisms underlying arsenic-induced carcinogenesis remain elusive. Here we show that chronic and low level of arsenic stress induces transformation of t...
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Chemical Cross-Linking and Mass Spectrometric Analysis of the Endogenous Yeast Exosome Complexes
Chemical cross-linking and mass spectrometric readout (CX-MS) has become a useful toolkit for structural analysis of protein complexes. CX-MS enables rapid detection of a larger number of cross-link peptides f...
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Using the Whole Cell Patch Clamp Technique to Study the Effect of Nanoparticles in Hippocampal Neurons
Several types of nanoparticles are considered toxic to the central nervous system. Patch-clamp is one of the most indispensable techniques in the study of neuroscience, especially in the field of neurophysiolo...
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Navigating Xenbase: An Integrated Xenopus Genomics and Gene Expression Database
Xenbase is the Xenopus model organism database (www.xenbase.org), a web-accessible resource that integrates the diverse genomic and biological data for Xenopus research....
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A Novel Colorimetric PCR-Based Biosensor for Detection and Quantification of Hepatitis B Virus
Hepatitis B virus (HBV) can cause viral infection that attacks the liver and it is a major global health problem that puts people at a high risk of death from cirrhosis of the liver and liver cancer. HBV has i...
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Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines
The retina is a thin neural tissue sitting on the backside of the eye, composed of light-sensing cells, interneurons, and output ganglion neurons. The latter send electrical signals to higher visual centers in...
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A Lateral Flow Biosensor for the Detection of Single Nucleotide Polymorphisms
A lateral flow biosensor (LFB) is introduced for the detection of single nucleotide polymorphisms (SNPs). The assay is composed of two steps: circular strand displacement reaction and lateral flow biosensor de...
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DNA G-Quadruplex-Based Assay of Enzyme Activity
DNA G-quadruplexes are special three-dimensional (3D) DNA nanostructures formed by specific G-rich DNA sequences. These 3D DNA nanostructures can bind with hemin and significantly improve the intrinsic peroxid...
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MicroRNAs, Long Noncoding RNAs, and Their Functions in Human Disease
Majority of the human genome is transcribed into RNAs with absent or limited protein-coding potential. microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) are two major families of the non-protein-coding tran...
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A Protocol to Collect Specific Mouse Skeletal Muscles for Metabolomics Studies
Due to the highly sensitive nature of metabolic states, the quality of metabolomics data depends on the suitability of the experimental procedure. Metabolism could be affected by factors such as the method of ...
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Erratum to: Genome-Wide Detection of Selection and Other Evolutionary Forces
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Genome-Wide Detection of Selection and Other Evolutionary Forces
As is well known, pathogenic microbes evolve rapidly to escape from the host immune system and antibiotics. Genetic variations among microbial populations occur frequently during the long-term pathogen–host ev...
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β-Actin in the Signaling of Transmembrane TNF-α-Mediated Cytotoxicity
To study the role of β-actin in the signaling of transmembrane TNF-α (tmTNF-α)-mediated cytotoxicity, we mainly used bioassay and apoptosis assay, detection of the visual changes in β-actin and intracellular t...
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Use of the Tumor Repressor DEDD as a Prognostic Marker of Cancer Metastasis
DEDD, a member of a family of death effector domain-containing proteins, plays crucial roles in mediating apoptosis, regulating cell cycle, and inhibiting cell mitosis. Our recent work demonstrates that DEDD i...
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Lateral Flow Biosensors for the Detection of Nucleic Acid
The detection of nucleic acid is of central importance for the diagnosis of genetic diseases, infectious agents, and biowarfare agents. Traditional strategies and technologies for nucleic acid detection are ti...
