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  1. No Access

    Protocol

    Yeast Display of Engineered Antibody Domains

    Yeast display is an efficient technology for selection of antibodies and other proteins with high affinity and thermal stability. Here, we describe a method for affinity maturation of engineered antibody domai...

    Qi Zhao, Zhongyu Zhu, Dimiter S. Dimitrov in Therapeutic Proteins (2012)

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    Protocol

    Construction of a Human Antibody Domain (VH) Library

    Highly diverse antibody (Fab or scFv) libraries have become vital sources to select antibodies with high affinity and novel properties. Combinatorial strategies provide efficient ways of creating antibody libr...

    Weizao Chen, Zhongyu Zhu, **aodong **ao, Dimiter S. Dimitrov in Therapeutic Antibodies (2009)

  3. No Access

    Protocol

    Construction of a Large Naïve Human Phage-Displayed Fab Library Through One-Step Cloning

    Antibody-based therapeutics is attracting more attention in the post-genome era, in contrast to a diminution in the initial high expectation for rapid development of gene-based therapeutic modalities. In suppo...

    Zhongyu Zhu, Dimiter S. Dimitrov in Therapeutic Antibodies (2009)