6 Result(s)
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Protocol
Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR/Cas9
Obtaining photoperiod-sensitive genic male sterility (PGMS) lines is one of the most important steps in two-line hybrid rice breeding. Traditionally, such lines were screened and developed with a classic rice ...
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Protocol
Identification of Phloem Mobile mRNAs Using the Solanaceae Heterograft System
Large numbers of mRNAs move in the phloem and some may function as signals to exert important physiological functions in the distal recipient organs. Generating an authentic list of phloem mobile mRNA is a pre...
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Protocol
Knocking Out MicroRNA Genes in Rice with CRISPR-Cas9
Background: MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in plant development and stress responses. Loss-of-function analysis of miRNA genes has been traditionally challenging due to lack...
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Protocol
Label-Free Quantitative Proteomics in Plant
Label-free quantitation (LFQ) proteomics, mainly based on the extraction of the peptide (precursor) intensity at the MS1 (mass spectrum 1) level, enables to quantify the relative amount of the proteins among samp...
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Protocol
Genetic, Epigenetic, and Environmental Control of Seed Dormancy and Germination
Seed germination is controlled by a combination of the seed dormancy level and environmental conditions such as light, temperature, moisture, and nitrate levels. Seed dormancy is programed genetically, but it ...
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Protocol
Assessment of Dormancy Level and Germination Ability of Seeds with Physiological Dormancy
Germination test is fundamental and commonly used technique for seed dormancy and germination studies, and proper assessment of dormancy level and germination ability of a given set of seeds is prerequisite fo...
