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Protocol
Genetic Engineering of a Phage-Based Delivery System for Endogenous III-A CRISPR-Cas System Against Mycobacterium tuberculosis
The increasing emergence of antibiotic-resistant bacteria makes it more difficult for us to fight against infectious diseases and develop new antibiotics. The CRISPR-Cas system, as a bacterial adaptive immune ...
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Protocol
Overview of Tandem Mass Spectral and Metabolite Databases for Metabolite Identification in Metabolomics
Liquid chromatography–mass spectrometry (LC-MS) is one of the most popular technologies in metabolomics. The large-scale and unambiguous identification of metabolite structures remains a challenging task in LC...
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Protocol
The Use of LipidIMMS Analyzer for Lipid Identification in Ion Mobility-Mass Spectrometry-Based Untargeted Lipidomics
Untargeted lipidomics aims to comprehensively measure and characterize all lipid species in biological systems. Ion mobility-mass spectrometry (IM-MS) has showed a great potential for untargeted lipidomic anal...
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Protocol
A High-Throughput Targeted Metabolomics Workflow for the Detection of 200 Polar Metabolites in Central Carbon Metabolism
Targeted metabolomics aims to analyze a set of pre-selected metabolites from biologically relevant metabolic pathways. The triple quadrupole mass spectrometry (QqQ-MS) based multiple reaction monitoring (MRM) ...