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  1. No Access

    Protocol

    CRISPR-Based Transcriptional Activation in Drosophila

    Overexpression is one of the classical approaches to study pleiotropic functions of genes of interest. To achieve overexpression, we often increase the transcription by introducing genes on exogenous vectors o...

    Yuting Han, **nyi Lu, Yutong Li, Yuhao Qiu, **zhi Dong, **aochen Li, Xu Si… in Drosophila (2022)

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    Protocol

    CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity

    A critical stage in performing gene editing experiments using the CRISPR/Cas9 system is the design of guide RNA (gRNA). In this chapter, we conduct a review of the current gRNA design rules for maximizing on-t...

    Kasidet Hiranniramol, Yuhao Chen, **aowei Wang in RNA Interference and CRISPR Technologies (2020)