9,319 Result(s)
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Protocol
Quantifying ABCA1/apoA-1 Signaling Pathways with AFM Imaging and Lipidomic Analysis
The role of lipid metabolic pathways in the pathophysiology of primary open-angle glaucoma (POAG) has been thoroughly elucidated, with pathways involved in lipid-related disorders such as hypercholesterolemia ...
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Protocol
Nanoparticles and Their Applications in Lipid Signaling
This chapter provides an overview of the diverse range of applications associated with nanoparticles. The application of nanoparticles in the medical field has garnered considerable attention due to their uniq...
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Protocol
Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria
In this chapter, we describe in detail how to perform a successful smFISH experiment and how to quantify mRNA transcripts in bacterial cells. The flexibility of the method allows for straightforward adaptation...
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Protocol
Measurement of Intracellular Ca2+ for Studying GPCR-Mediated Lipid Signaling
Intracellular Ca2+ can be conveniently monitored by sensitive Ca2+ fluorescent dyes in live cells. The Gαq involved lipid signaling pathways and, thus, can be studied by intracellular Ca2+ imaging. Here we descri...
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Protocol
RNA and Protein Detection by Single-Molecule Fluorescent in Situ Hybridization (smFISH) Combined with Immunofluorescence in the Budding Yeast S. cerevisiae
The inherent stochastic processes governing gene expression give rise to heterogeneity across individual cells, highlighting the importance of single-cell studies. The emergence of single-molecule fluorescent ...
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Protocol
Effects of a Laparotomy on Targeted Lipidomics Profiles in a Mouse Model of Surgical Stress During Aging
Laparotomy (EL) is one of the most common procedures performed among surgical specialties. Previous research demonstrates that surgery is associated with an increased inflammatory response. Low psoas muscle ma...
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Protocol
In Vivo Production of dsRNA Using Bacteriophage ϕ6 in Pseudomonas syringae Cit7 Cells
RNA interference (RNAi), also known as post-transcriptional gene silencing (PTGS), is one of the emerging genetic engineering techniques to effectively silence or inhibit the expression of target genes. This c...
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Protocol
SABER-FISH in Hydractinia
In situ hybridization allows the detection of nucleic acid sequences in fixed cells and tissues. The gelatinous nature of cnidarians and Hydractinia demands extensive and exhausting protocols to detect RNA transc...
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Protocol
Detection of dsRNA by Acridine Orange Staining
Acridine orange is a nucleic acid binding dye that emits green fluorescence when bound to double-stranded DNA or RNA and red fluorescence when bound to single-stranded DNA or RNA under ultraviolet light. This ...
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Protocol
Transiently Induce RNA Silencing in Plants Using a Tobacco Necrosis Virus A (TNV-A)-Based dsRNA Production System
Transgenic expression of hairpin RNA or artificial microRNA is widely used for genetic studies in plant science. However, induction of RNA silencing by transgenic method may have a problem when studying essent...
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Protocol
Fluorescent In Situ Detection of Small RNAs in Plants Using sRNA-FISH
Plant small RNAs are 21–24 nucleotide, noncoding RNAs that function as regulators in plant growth and development. Colorimetric detection of plant small RNAs was made possible with the introduction of locked n...
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Protocol
Rapid Purification of dsRNA Using Micro-Spin Cellulose Column
Double-stranded RNA (dsRNA) is the replication intermediates of all RNA viruses. Purification and analysis of the profile and sequence of dsRNA is vital in virus diagnoses and/or characterization. Cellulose is...
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Protocol
Analysis of Plant Virus-Induced Immunity by Using Viral-Derived Double-Stranded RNA in Arabidopsis thaliana
Pattern-triggered immunity is the first line of defense against infection by pathogens such as bacteria and fungi in plants, and this mechanism remains poorly defined in plant viruses. Double-stranded RNA (dsR...
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Protocol
High-Throughput RNA-HCR-FISH Detection of Endogenous Pre-mRNA Splice Variants
RNA-fluorescence in situ hybridization (RNA-FISH) is an essential and widely used tool for visualizing RNA molecules in intact cells. Recent advances have increased RNA-FISH sensitivity, signal detection effic...
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Protocol
Tree Genome Databases: A New Era in the Development of Cyber-Infrastructures for Forest Trees
Genomic research is one of the important approaches for sustainable forest management to enhance and mitigate the essential human needs from the forests. However, the genomic researches on the forest tree spec...
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Protocol
Multiparametric Analysis of Apoptosis by Flow Cytometry
Flow cytometry remains the most widely used method for detecting and quantifying apoptosis and related forms of cell death in mammalian cells. The multiparametric nature of flow cytometry allows multiple apopt...
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Protocol
Detection of dsRNA with Fluorescence In Situ Hybridization (FISH)
Fluorescence in situ hybridization (FISH) is a powerful technique used for detecting and localizing specific nucleic acid sequences in cells or tissues. Double-stranded RNA (dsRNA) is a type of RNA with comple...
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Protocol
Production of Double-Stranded RNA in Planta by a Potato Mop-Top Virus (PMTV)-Based Vector for Inducing Gene Silencing
RNA silencing (also known as gene silencing) is an evolutionary conserved mechanism that is involved in regulating gene expression, suppressing mobile elements, and defensing virus infection. RNA silencing is ...
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Protocol
Artificial Intelligence in Genomic Studies
The presented chapter sheds some insight on some of the recent Artificial Intelligence (AI) based breakthroughs in the field of genomics. First section of the chapter gives brief overview of some of the most e...
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Protocol
CRISPR-Cas9-Mediated Bioluminescent Tagging of Endogenous Proteins by Fluorescent Protein-Assisted Cell Sorting
Oncogenic fusion genes are attractive therapeutic targets because of their tumor-specific expression and central “driver” roles in various human cancers. However, oncogenic fusions involving transcription fact...
