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Protocol
Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometry
Phospholipid fatty acid (FA) composition influences the biophysical properties of the plasma membrane and plays an important role in cellular signaling. Our previous work has demonstrated that plasma membrane ...
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Protocol
CdS Quantum Dots Modified Photoelectrochemical Biosensor for TATA-Binding Protein Probing
The photoelectrochemical (PEC) biosensor, in which light is utilized to excite the photoactive species and current is employed as the detection signal, is a newly appeared yet dynamically develo** technique ...
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Protocol
Murine Colonic Organoid Culture System and Downstream Assay Applications
Colonic organoids, three-dimensional colonic crypts grown in vitro that show realistic microanatomy, have many potential applications for studying physiology, developmental biology, and pathophysiology of inte...
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Protocol
Characterization of Long Noncoding RNA-Associated Proteins by RNA-Immunoprecipitation
With the advances in sequencing technology and transcriptome analysis, it is estimated that up to 75 % of the human genome is transcribed into RNAs. This finding prompted intensive investigations on the biolog...
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Protocol
Capillary Electrophoretic Enzyme Assays
In the past years, capillary electrophoresis has become a frequently used technique for enzyme assays due to the high separation efficiency and versatility as well as small sample size and low consumption of c...
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Protocol
Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technology
Congestive heart failure (CHF) is characterized by impaired cardiovascular reflexes and increased neurohumoral drive. The long-term sympatho-excitation increases the progression and risk of mortality during CH...
