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Protocol
3D-Autologous Culture Method for Precision Oncology
The 3D-autologous culture method (3D-ACM) for patient-derived cancer samples utilizes a patient’s own body fluid or serum to prepare a 3D scaffold and for the culture medium. 3D-ACM enables tumor cells and/or ...
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Protocol
The Detection of Exosomal PD-L1 in Peripheral Blood
Peripheral blood is a source for liquid biopsy, which can meet the requirements of pretreatment disease ty** to determine precise targeted therapy and monitoring of posttreatment minimal residual disease mon...
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Protocol
Functional Interrogation of Ca2+ Signals in Human Cancer Cells In Vitro and Ex Vivo by Fluorescent Microscopy and Molecular Tools
Genetically encoded calcium indicators (GECIs) and high-resolution confocal microscopy enable dynamic visualization of calcium signals in cells and tissues. Two-dimensional and 3D biocompatible materials mimic...
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Protocol
Develo** and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell Tumors
Germ cell tumors (GCTs) are a rare disease, but they account for 15% of all malignancies diagnosed during adolescence. The biological mechanisms underpinning their development are only starting to be explored....
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Protocol
Assessing Neurological Function in Brain Tumor Mouse Model
Of all cancer types, brain cancers often constitute the most devastating and difficult-to-treat tumors. In the United States, brain cancer accounts for 1 in every 100 cancer diagnoses. Although significant adv...
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Protocol
Deep Sequencing Reveals a MicroRNA Expression Signature in Triple-Negative Breast Cancer
Deep sequencing is an advanced technology in genomic biology to detect the precise order of nucleotides in a strand of DNA/RNA molecule. The analysis of deep sequencing data also requires sophisticated knowled...
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Protocol
LC-MS-Based Metabolomic Investigation of Chemopreventive Phytochemical-Elicited Metabolic Events
Phytochemicals are under intensive investigation for their potential use as chemopreventive agents in blocking or suppressing carcinogenesis. Metabolic interactions between phytochemical and biological system ...
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Protocol
Autoantibodies Against Cancer Antigens
With the identification of tumor antigens, the host immune response to various types of cancers can now be studied with a high degree of specificity in large cohorts of patients, in the hope of correlating imm...