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Protocol
Determination of Isomeric Glycan Structures by Permethylation and Liquid Chromatography–Mass Spectrometry (LC-MS)
The existence of glycans in isomeric forms is responsible for the multifariousness of their properties and biological functions. Their altered expression has been associated with various diseases and cancers. ...
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Protocol
Glycan Microarray Screening Assay for Glycosyltransferase Specificities
Glycan microarrays represent a high-throughput approach to determining the specificity of glycan-binding proteins against a large set of glycans in a single format. This chapter describes the use of a glycan m...