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Protocol
Using Microscopy Tools to Visualize Autophagosomal Structures in Plant Cells
Macroautophagy (hereafter as autophagy), is a metabolic process for sequestration of cytoplasmic cargos into a double membrane structure named as autophagosome. In plants, autophagy is required for nutrition m...
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Protocol
MicroRNAs: Biomarkers, Diagnostics, and Therapeutics
MicroRNAs (miRNAs) are small noncoding RNAs (21–23 nucleotides in length) that regulate gene expression at translational or posttranslational levels. The major regulatory mechanisms include translational repre...
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Protocol
Three Dimensional Cell Culture of Human Neural Stem Cells Using Polysaccharide-Based Hydrogels and Subsequent Bioanalyses
Protocols for culturing neural stem cells (NSCs) are increasingly finding utilization for studying and growing of tissues that can appropriately model the neural regeneration processes. Two-dimensional (2D) pl...