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    Protocol

    Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses

    Primary neuronal cell culture preparations are widely used to investigate synaptic functions. This chapter describes a detailed protocol for the preparation of a neuronal cell culture in which giant calyx-type...

    Dimitar Dimitrov, Laurent Guillaud, Kohgaku Eguchi… in Neurotrophic Factors (2018)

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    Protocol

    Patch-Clamp Recording Method in Slices for Studying Presynaptic Mechanisms

    The synapse plays pivotal roles in various neuronal functions, with its efficacy determining the opening and closing of neuronal circuits. Among the parameters that determine synaptic efficacy, presynaptic fac...

    Tomoyuki Takahashi, Tetsuya Hori, Yukihiro Nakamura… in Patch Clamp Techniques (2012)