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    Protocol

    CRISPR-Cas9-Mediated Genome Editing in Escherichia coli Bacteriophages

    Traditional low-efficiency homologous recombination between plasmids and phage genome was developed to generate recombinant phages with gene replacement, deletion, or insertion. Here, we describe a protocol fo...

    Yibao Chen, **angmin Li, Shuang Wang, ** Qian, Yingjun Li in CRISPR-Cas Methods (2021)

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    Protocol

    Detection of Epigenetic Field Defects Using a Weighted Epigenetic Distance-Based Method

    Identifying epigenetic field defects, notably early DNA methylation alterations, is important for early cancer detection. Research has suggested these early methylation alterations are infrequent across sample...

    Ya Wang, Min Qian, Peifeng Ruan… in Stem Cell Transcriptional Networks (2020)

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    Protocol

    Microfluidic Device Fabrication by Thermoplastic Hot-Embossing

    Due to their low cost compatibility with replication-based fabrication methods, thermoplastics represent an exceptionally attractive family of materials for the fabrication of lab-on-a-chip platforms. A divers...

    Shuang Yang, Don L. DeVoe in Microfluidic Diagnostics (2013)