6 Result(s)
within
Protocol
Rong-li **e
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Protocol
Automated Real-Time Monitoring of Extracellular pH to Assess Early Plant Defense Signaling
Extracellular alkalinization mediated by the inhibition of plasma membrane-located proton pum** ATPases hallmarks the initiation of defense signaling in plant cells. Early defense responses also include depo...
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Protocol
Quantitative Measurement of Pattern-Triggered ROS Burst as an Early Immune Response in Tomato
The rapid accumulation of extracellular “reactive oxygen species” (ROS), also known as the “oxidative burst”, is an early plant immune response triggered by pathogen-derived microbe-associated molecular patter...
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Protocol
Homogeneous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells
Hepatocyte-like cells (HLCs) generated from human induced pluripotent stem cells (iPSCs) could provide an unlimited source of liver cells for regenerative medicine, disease modeling, drug screening, and toxico...
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Protocol
Production of Genetically Engineered Porcine Embryos by Handmade Cloning
Genetic engineering is essential to realize the full potentials of pigs both as livestock and as animal models of human disease. With the development of new genetic engineering technologies, such as the cluste...
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Protocol
Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins
The expression analysis of recombinant proteins is a challenging step in any high-throughput protein production pipeline. Often multiple expression systems and a variety of expression construct designs are con...
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Protocol
Fluorescence Fluctuation Spectroscopy and Imaging Methods for Examination of Dynamic Protein Interactions in Yeast
Protein interactions are inherently dynamic. In no system is this more true and important than in signaling pathways, where spatial and temporal control of specific protein interactions is key to signaling spe...
