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    Protocol

    Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay

    Western blotting has proven to be an important technique in analysis of receptor-ligand interactions (i.e., by ligand blotting) and for identifying molecules mediating cell attachment (i.e., by cell blotting)....

    Robert Sackstein, Robert Fuhlbrigge in Protein Blotting and Detection (2009)

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    Protocol

    The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions

    Adhesive interactions of cells with blood vessel walls under flow conditions are critical to a variety of processes, including hemostasis, leukocyte trafficking, tumor metastasis, and atherosclerosis. We have ...

    Robert Sackstein, Robert Fuhlbrigge in Cell-Cell Interactions (2006)