12 Result(s)
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Protocol
Qing-**ang Wang
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Protocol
High-Throughput Single-Nucleotide Polymorphisms Genoty**
Single-nucleotide polymorphisms (SNPs) are DNA sequence variations that occur at a single base in the genome sequence. SNPs are valuable markers for identifying genes responsible for susceptibility to common d...
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Protocol
Proteomics With Two-Dimensional Gel Electrophoresis and Mass Spectrometry Analysis in Cardiovascular Research
Proteomics is a large-scale, comprehensive study of the proteins of a cell or organism. It is a unique means of characterizing proteins that are expressed in a cell or tissue at any given time-point and of ide...
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Protocol
SAGE Programs
A complex disease trait refers to a phenotype that does not follow simple Mendelian segregation attributable to a single gene locus, but instead may be caused by multiple disease loci, their interactions, poly...
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Protocol
Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis
Cytoplasma is the cell interior place between the cellular membrane and the nucleus, where various intracellular activities take place, including energy production, reactive oxygen species (ROS) detoxification...
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Protocol
The TaqMan Method for SNP Genoty**
Single nucleotide polymorphisms (SNPs) are common DNA sequence variations that occur at single bases within the genome. SNPs have been instrumental in elucidating the genetic basis of common, complex diseases ...
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Protocol
Identification and Expression Analysis of Long Intergenic Noncoding RNAs
Long intergenic noncoding RNAs (lincRNAs) have caught increasing attention in recent years. The advance of RNA-Seq has greatly facilitated the discovery of novel lincRNAs. However, the computational analysis o...
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Protocol
Characterization of Flowering Time Mutants
Timing of flowering, which is adapted to the ambient environment, is one of the key traits to ensure the reproductive success of plants. Our current understanding of the complex genetic control network of this...
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Protocol
Autophagy in Platelets
Anucleate platelets are produced by fragmentation of megakaryocytes. Platelets circulate in the bloodstream for a finite period: upon vessel injury, they are activated to participate in hemostasis; upon senesc...
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Protocol
Multiple Target Drug Design Using LigBuilder 3
Designing drugs that directly interact with multiple targets is a promising approach for treating complicated diseases. In order to successfully bind to multiple targets of different families and achieve the d...
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Protocol
Profiling Chromatin Landscape at High Resolution and Throughput with 2C-ChIP
Chromatin immunoprecipitation (ChIP) is used to probe the presence of proteins and/or their posttranslational modifications on genomic DNA. This method is often used alongside chromosome conformation capture a...
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Protocol
Analysis of Actin Array Rearrangement During the Plant Response to Bacterial Stimuli
Plants are constantly exposed to various environmental stresses, among which, microbial pathogens are one of the major threats. Studies have shown that the host actin cytoskeleton undergoes active rearrangemen...
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Protocol
Dissecting the Role of the Ccr4–Not Deadenylase Complex in Pluripotency and Differentiation
In this chapter, we describe methods and assays to examine the role of the Ccr4–Not mRNA deadenylase complex in regulating mouse embryonic stem cell (ESC) pluripotency and differentiation. We present the follo...
