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Profiling Chromatin Landscape at High Resolution and Throughput with 2C-ChIP
Chromatin immunoprecipitation (ChIP) is used to probe the presence of proteins and/or their posttranslational modifications on genomic DNA. This method is often used alongside chromosome conformation capture a...
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Protocol
Dissecting the Role of the Ccr4–Not Deadenylase Complex in Pluripotency and Differentiation
In this chapter, we describe methods and assays to examine the role of the Ccr4–Not mRNA deadenylase complex in regulating mouse embryonic stem cell (ESC) pluripotency and differentiation. We present the follo...