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Protocol
Genetic Modification of Murine Dendritic Cells by RNA Transfection
The ability to manipulate in vitro cultured dendritic cells (DCs) by transfection represents an attractive strategy to load these antigen-presenting cells with genetic material encoding various immunogenic epi...
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Protocol
Purification and Characterization of MHC Class II Containing Organelles in Mouse Bone-Marrow-Derived Dendritic Cells
The initiation and propagation of the immune responses is dependent on the ability of antigen-presenting cells (APC) to convert proteins into peptides, to load them intracellularly onto major histocompatibilit...