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    Protocol

    Top-Down Proteomics by Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Large-Scale Characterization of Proteoforms in Complex Samples

    Capillary zone electrophoresis (CZE) is a fundamentally simple and highly efficient separation technique based on differences in electrophoretic mobilities of analytes. CZE-mass spectrometry (MS) has become an...

    Elijah N. McCool, Rachele A. Lubeckyj… in Capillary Electrophoresis-Mass Spectrometr… (2022)

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    Protocol

    Flow Cytometry-Based Detection of Siglec Ligands

    Siglecs are a family of immunomodulatory cell surface receptors present on white blood cells. Binding to cell surface sialic acid-containing glycans modulates the proximity of Siglecs to other receptors that t...

    Edward N. Schmidt, Jaesoo Jung, Matthew S. Macauley in Carbohydrate-Protein Interactions (2023)

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    Protocol

    Qualitative and Quantitative Characterization of Protein–Carbohydrate Interactions by NMR Spectroscopy

    Solution-state nuclear magnetic resonance (NMR) spectroscopy can be used to monitor protein–carbohydrate interactions. Two-dimensional 1H-15N heteronuclear single quantum coherence (HSQC)-based techniques describ...

    Julie M. Grondin, David N. Langelaan, Steven P. Smith in Carbohydrate-Protein Interactions (2023)