6 Result(s)
within
Protocol
Miaomiao **n
English
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Protocol
Studies on Protein–RNA:DNA Hybrid Interactions by Microscale Thermophoresis (MST)
Microscale thermophoresis (MST) is a technology that allows for quantitative analysis of interactions between biomolecules with low sample consumption. MST uses localized temperature fields to measure the diff...
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Protocol
DNA Affinity Purification Sequencing (DAP-Seq) for Map** Genome-Wide Transcription Factor Binding Sites in Plants
DNA affinity purification sequencing (DAP-seq) couples in vitro expression of transcription factor (TF) and binding to a genomic DNA library with next-generation sequencing, providing a robust method to identi...
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Protocol
Telomere and G-Quadruplex Colocalization Analysis by Immunofluorescence Fluorescence In Situ Hybridization (IF-FISH)
Four-stranded G-quadruplexes consists of tracks of guanines that are stabilized by Hoogsteen base pairing. The formation of G-quadruplexes in genomic DNA contribute to numerous biological processes in vivo, in...
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Protocol
ARGONAUTE Genes in Salvia miltiorrhiza: Identification, Characterization, and Genetic Transformation
Small RNA-mediated gene silencing is a vital regulatory mechanism in eukaryotes that requires ARGONAUTE (AGO) proteins. Salvia miltiorrhiza is a well-known traditional Chinese medicinal plant. Therefore, it is im...
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Protocol
Extraction Methods of Natural Products from Traditional Chinese Medicines
In recent years, many research activities have focused on Natural Products (NPs) derived from Traditional Chinese medicines (TCMs), thus making a renaissance in the drug discovery process of TCMs. Maximizing t...
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Protocol
Colorimetric Detection of PNA-DNA Hybridization Using Cyanine Dyes
The high affinity and sequence selectivity with which peptide nucleic acid (PNA)oligomers bind to complementary DNA and RNA makes them attractive hybridization probes for diagnostics. As is the case for all de...
