12 Result(s)
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Protocol
Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects
Since this book was originally published in 2007 there has been a significant increase in the number of Salmonella bacteriophages, particularly lytic virus, and Salmonella strains which have been fully sequenc...
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Protocol
Targeting Brain Tumor Stem Cells with Oncolytic Adenoviruses
In 2004, brain tumor stem cells (BTSCs) were isolated from surgical human malignant gliomas. This cancer cell population has been identified as the root for tumor initiation and resistance to therapies. Thus, ...
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Protocol
Construction of Targeted and Armed Oncolytic Adenoviruses
Oncolytic (replication-competent) adenoviruses (Ads) represent the most advanced platform for cancer gene therapy. These viral vectors ablate tumors by killing tumor cells in the process of virus replication. ...
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Protocol
The Development of Gene Therapy: From Monogenic Recessive Disorders to Complex Diseases Such as Cancer
During the last 4 decades, gene therapy has moved from preclinical to clinical studies for many diseases ranging from monogenic recessive disorders such as hemophilia to more complex diseases such as cancer, c...
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Protocol
A Real-Time Taqman® Method for Hepatitis C Virus Genoty** and Methods for Further Subty** of Isolates
The HCV genome is highly heterogeneous; more and more genotypes, each with several distinct subtypes, are being identified around the world. Knowledge of genotype is important for planning of treatment regimes...
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Protocol
Selection and Characterization of Drug-Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay
Because HCV RNA-dependent RNA polymerase is error-prone and the viral RNA has a high turnover rate, the genetic diversity of HCV is very high both in vitro and in vivo. The mutation rate in long-term replicon cul...
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Protocol
In Vivo Study of HCV in Mice with Chimeric Human Livers
Estimates of hepatitis C virus infection include 170 million people worldwide, who face increased risk of development of cirrhosis, liver failure, and hepatocellular carcinoma. Standard of care therapy with pe...
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Protocol
Cellular Receptors and HCV Entry
After attachment to specific receptors on the surfaces of target cells, hepatitis C virus (HCV) particles are thought to be internalized to endosomes, where low pH induces fusion between the viral and cellular...
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Protocol
Magnetic Resonance Microscopy of Mouse Brain Development
Magnetic resonance imaging (MRI) is increasingly becoming an important tool to study anatomy of rodent brains. Compared with histology, it has clear advantages because the entire 3D object can be captured as a...
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Protocol
Construction of VNP20009
The engineering of tumor-targeting Salmonella strains bearing a high degree of both attenuation and tumor inhibition is a recently developed approach to vector-based treatment of cancer (1,2). Tumor-targeted Salm...
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Protocol
Chronic Intracerebral Delivery of Trophic Factors via a Programmable Pump as a Treatment for Parkinsonism
The most common treatment for Parkinson’s disease (PD) aims at pharmacologically augmenting striatal dopamine (DA) using the DA precursor levodopa. Such treatment provides symptomatic relief, but does not slow...
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Protocol
Intraocular Delivery of Recombinant Virus
In the past 5 yr, advances in technology have been made that allow efficient somatic transfer of functional genes to target cells in the eye in vivo. The ability to deliver functional genes to these cells is d...
