32 Result(s)
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Protocol
Isolation of Mitochondria from Murine Skeletal Muscle
Skeletal muscle is one of the largest tissues in human body. Besides enabling voluntary movements and maintaining body’s metabolic homeostasis, skeletal muscle is also a target of many pathological conditions....
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Protocol
Visualizing Chromatin Modifications in Isolated Nuclei
Modifications in chromatin structure are traditionally monitored by biochemical assays that provide average measurements of static events in a population of cells. Microscopy provides a method by which single ...
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Protocol
Isolation and Culture of Embryonic Pancreas and Liver
Culturing embryonic tissue in an in vitro setting offers the unique ability to manipulate the external medium and therefore to investigate the pathways involved in regulating normal organogenesis as well as pr...
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Protocol
Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential
Sequences with the potential to form intramolecular G-quadruplexes (G4-structures) are found in highly nonrandom distributions in the genomes of diverse organisms. These sequences are associated with nucleic a...
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Protocol
Application of Cell Traction Force Microscopy for Cell Biology Research
When a cell migrates, it generates traction forces on the underlying substrate. The cell traction force (CTF) is not only essential for cell migration, but it is also used by cells to control their shape and m...
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Protocol
Lipid Raft-Redox Signaling Platforms in Plasma Membrane
Membrane lipid rafts (LRs) have been demonstrated to be importantly involved in transmembrane signaling in a variety of mammalian cells. Many receptors can be aggregated within the LR clusters to form signalin...
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Protocol
Functional Assays for Hematopoietic Stem Cell Self-Renewal
Stem cells are defined by the ability to self-renew. Specific functional assays have been developed for the rigorous identification and quantification of hematopoietic stem cells (HSCs), making these cells the...
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Protocol
Isolation and Culture of Adult Mouse Hepatocytes
The liver performs a multitude of functions including the regulation of carbohydrate, fat, and protein metabolism, the detoxification of endo- and xenobiotics, and the synthesis and secretion of plasma protein...
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Protocol
Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches
Although embryonic stem cells (ESCs) have been established from mice since 1981, attempts to derive its counterparts from various other mammals, including rats, have not succeeded. Recently, induced pluripoten...
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Protocol
Murine Hematopoietic Stem Cell Transduction Using Retroviral Vectors
Hematopoietic stem cells (HSCs) represent an important target cell population in bone marrow transplantation and gene therapy applications. Their progeny cells carry the genetic information of the HSCs and rep...
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Protocol
Leukemia Diagnosis in Murine Bone Marrow Transplantation Models
The mouse is the most commonly used experimental animal, and a wide range of tumor types can arise in their hematopoietic system. Therefore, for research scientists and graduate students working in the field ...
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Protocol
Analysis of Microdissected Cells by Two-Dimensional LC-MS Approaches
Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. We combined the ...
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Protocol
Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo
The mammalian two-hybrid system is a very powerful tool to investigate protein-protein interactions in terms of functional domains and identify potential binding ligands and partners of a protein. Compared wit...
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Protocol
Delivery of DNA into Tumors
Delivery of plasmid DNA encoding therapeutic genes into tumors is one of the main applications of electroporation. This chapter summarizes various investigators' electroporation parameters for intratumoral gen...
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Protocol
Systemic IL-12 Gene Therapy for Treating Malignancy via Intramuscular Electroporation
Interleukin 12 (IL-12) is effective in treating systemic microscopic malignancies by inducing T helper 1 (TH1) response, inhibiting angiogenesis, and triggering secondary cytokine production. Unfortunately, daily...
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Protocol
Analysis of Receptor Tyrosine Kinase Internalization Using Flow Cytometry
The internalization of activated receptor tyrosine kinases (RTKs) by endocytosis and their subsequent down regulation in lysosomes plays a critical role in regulating the duration and intensity of downstream s...
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Protocol
Electron Spectroscopic Imaging of the Nuclear Landscape
Our understanding of sub-nuclear organisation is largely based on fluorescence and electron microscopy methods. Conventional electron microscopy, which depends on heavy atom contrast agents, provides excellent...
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Protocol
Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumors in Dogs
Bleomycin and Interleukin 12 have been used clinically to treat tumors; however, the co-administration of Bleomycin and Interleukin 12 followed by electroporation has not been tested clinically. In this study,...
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Protocol
Treatment of SCCVII Tumors with Systemic Chemotherapy and Interleukin-12 Gene Therapy Combination
Cyclophosphamide and Interleukin-12 (IL-12) have been successfully used in clinical trials for treating malignancies. In this study, we explore the coadministration of cyclophosphamide and IL-12 plasmid DNA fo...
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Protocol
Reducing Sample Complexity in Proteomics by Chromatofocusing with Simple Buffer Mixtures
Chromatofocusing has many potential applications in the field of proteomics, such as for the isolation and removal of major sample components to facilitate the analysis of low-abundance components, and for sam...
